新疆维吾尔族MT1XT20基因与微卫星不稳定结直肠癌相关性研究  

The association study between Xinjiang Uygur people MT1XT20 gene and microsatellite instability colorectal cancer

作　　者：吴静[1,2] 龚旭晨[1] 孔长青[2] 赵宗峰[3] 

机构地区：[1]安徽医科大学新疆临床学院(新疆维吾尔自治区人民医院)肛肠外科,乌鲁木齐830001 [2]安徽医科大学新疆临床学院(新疆维吾尔自治区人民医院)肝胆血管外科,乌鲁木齐830001 [3]安徽医科大学新疆临床学院(新疆维吾尔自治区人民医院)临床试验中心,乌鲁木齐830001

出　　处：《安徽医科大学学报》2015年第6期867-869,共3页Acta Universitatis Medicinalis Anhui

基　　金：新疆维吾尔自治区人民医院院内基金(编号:20130315)

摘　　要：检测临床手术切除43例新疆维吾尔族结直肠癌患者癌组织及相应癌旁正常组织中微卫星不稳定(MSI)状态,探讨MT1XT20基因与新疆维吾尔族MSI结直肠癌发生的关系。通过聚合酶链反应-单链构象多态性(PCR-SSCP)技术对新疆维吾尔族MT1XT20基因与MSI结直肠癌检测及分析。在43例新疆维吾尔族患者中24例癌组织发生微卫星改变,总检出率为55.81%;8例发生一个微卫星位点改变,两个以上位点改变的有16例,癌旁正常组织中未检测到微卫星改变。在MT1XT20位点上存在较高频率的微卫星改变,提示可能存在MT1XT20基因与新疆维吾尔族MSI结直肠癌发病密切相关,为MSI结直肠癌的早期诊断提供了有意义的检测指标。The microsatallite（MSI）stability of cancer and adjacent normal tissues was detected in 43 cases of Xinjiang Uygur patients with colorectal cancer after resection and the relationship between the MT1XT20 gene and the incidence of MSI colorectal cancer in Xinjiang Uygur people was discussed. The polymerase chain reaction -single strand conformation polymorphism（ PCR-SSCP）technique was used for the detection and analysis of the Xinjiang Uygur MT1XT20 gene and MSI colorectal cancer. In 43 Xinjiang Uygur patients,24 cases had microsatel-lite alterations,with the total detection rate of 55. 81% ,8 cases sent a microsatellite alteration and 16 cases had more than two locus alterations. However,no microsatellite alteration was found in adjacent normal tissues. There was a high frequency of microsatellite alteration at the MT1XT20 locus,suggesting a close relationship between MT1XT20 gene and the incidence of colorectal cancer in Xinjiang Uygur people,thus providing a significant meas-ure for the early diagnosis of MSI colorectal cancer.

关 键 词：结直肠癌 微卫星不稳定 MT1XT20基因 

分 类 号：R394-33[医药卫生—医学遗传学]