Bio-oss骨粉复合HIF-1α蛋白对拔牙创骨愈合影响的实验研究  

An Experimental Study of Bio-oss with HIF-1α Protein on Tooth Extraction Bone Healing

作　　者：谭鸾君[1] 阮征[1] 张劲娥[1] 黄远亮[1] 王磊[1] 

机构地区：[1]同济大学附属东方医院口腔科,上海200120

出　　处：《口腔医学研究》2015年第5期441-443,共3页Journal of Oral Science Research

基　　金：浦东新区卫生局青年项目(PW2011B-13);东方启航计划面上项目(DFQH-M101);浦东新区科技发展基金创新资金(PKJ2013-Y15)

摘　　要：目的:研究Bio-oss骨粉复合HIF-1α蛋白对拔牙创骨愈合的影响。方法:全麻下完整拔除3只Beagle犬的双侧下颌二、四前磨牙近中根,随机选择一个牙槽窝作为空白对照组,其余即刻分别植入bio-oss骨粉、biooss复合浓度为100μg/L的HIF-1α蛋白,分别与术后6、12周取材,进行Micro-CT扫描、硬组织切片染色,观察拔牙创的骨愈合情况和组织病理学变化。结果:Micro-ct扫描显示,第6周时各组骨小梁数目、骨密度和BV/TV均有一定的差别。第12周时,bio-oss复合浓度HIF-1α组与bio-oss骨粉组、空白组差别显著(P<0.05),差别有统计学意义。硬组织切片显示第12周时,bio-oss复合HIF-1α组有良好的新骨形成。结论:Bio-oss骨粉复合HIF-1α蛋白骨修复材料可以促进拔牙创的愈合。Objective：To study Bio-oss bone with HIF-1αprotein on tooth extraction bone healing.Methods：Under general anesthesia,mesial root of the 2nd,4th premolars in both sides of mandible of 3Beagle dogs were extracted.Randomly,a wound was selected as the blank control group,and the rest were immediately implanted biooss,bio-oss with HIF-1αprotein of 100ng/ml.After six and twelve weeks,the beagle dogs were sacrificed.The gross speciments were examined by Micro-CT images,and mineralized specimen preparations were prepared.Results：Micro-CT scan analysis showed that each group had some differences in the number of trabecular,bone mineral density and BV/TV.After twelve weeks,bio-oss with HIF-1αgroup was significantly higher than the biooss group、the control group（P〈0.05）.The difference was statistical significance.Tissue slides also revealed an effective osteogensis in those wounds implanted with bio-oss with HIF-1α.Conclusion：Bio-oss with HIF-1αprotein l can promote the regeneration of alveolar bone.

关 键 词：BIO-OSS骨粉 HIF-1Α蛋白 拔牙创 骨愈合 

分 类 号：R782.1[医药卫生—口腔医学]