表没食子儿茶素没食子酸酯对HPV11早期基因E6、E7mRNA表达的影响  

Effect of(-)- epigallocatechin-3- gallate on Expression of HPV11 E6 and E7 mRNA

作　　者：孙洋[1] 李新宇[1] 宋莎莎[1] 王永芳[1] 顾恒[1] 

机构地区：[1]中国医学科学院/北京协和医学院皮肤病研究所,南京210042

出　　处：《医学研究杂志》2015年第5期74-77,共4页Journal of Medical Research

基　　金：中国医学科学院/北京协和医学院协和青年基金资助项目(3332013016)

摘　　要：目的利用人工构建的含人乳头瘤病毒（HPV）11型基因组的HaCaT细胞（简称为HPVll．HaCaT细胞），研究表没食子儿茶素没食子酸酯（EGCG）对该细胞生长的抑制作用，以及受试物对HPVll早期基因E6和E7mRNA表达的影响。方法在HaCaT及HPVll．HaCaT细胞培养基中添加不同浓度的EGCG后，应用MTT比色法检测受试物对细胞生长增殖的影响；用相对荧光定量PCR法检测HPVll．HaCaT细胞中HPVllE6、E7mRNA表达的改变。结果EGCG能抑制HaCaT和HPVll．HaCaT细胞的生长，不同浓度EGCG作用下，细胞生存率为52％-95％、64％～90％，呈浓度和时间依赖趋势。EGCG作用于细胞12h和24h后，HPVll．HaCaT细胞中HPVllE6、E7基因的mRNA表达水平明显下降（P〈0．05）。结论EGCG在体外能抑制含HPVll基因组的HaCaT细胞生长，并能抑制HPVll功能基因E6、E7表达。Objective Using of the HaCaT cells which contain the whole genes of human papillomavirus type 11 （ HPV11. HaCaT）, to study the effect of （ - ） - epigalloeateehin - 3 - gallate （EGCG） on cell proliferation and expression of HPVII E6 and E7 mRNA. Methods The HaCaT and HPV11. HaCaT cells were cultured and treated with different concentration of EGCG for different periods of time. The effects on proliferation of HaCaT and HPV11. HaCaT cells were examined by MTT assay. The alterations of HPVI1 E6 and E7 mRNA level were examined by real - time fluorescence quantitative PCR. Results EGCG can inhibit the proliferation of HaCaT and HPVI 1. HaCaT in a dose - and time -dependent. EGCG in different concentration resulted in the cell survival rate ranging from 52% to 95% and 64% to 90%. After incubating the ItPVll. HaCaT cells with EGCG for 12h and 24h, the expression of HPVll E6 and E7 mR- NA is inhibited obviously （P 〈 0.05）. Conclusion EGCG can inhibit the proliferation of HPVll. HaCaT cells and the expression of HPVll E6 and E7 mRNA in vitro.

关 键 词：尖锐湿疣 人乳头瘤病毒11型 表没食子儿茶素没食子酸酯 E6 E7 

分 类 号：R282.71[医药卫生—中药学]