鼻咽癌患者血浆miR-24异常表达的临床意义  

Clinical significance of plasma miR-24 dysregulation in nasopharyngeal carcinoma

作　　者：王路[1] 余伯龙[1] 岑建华[1] 彭新宇[1] 刘友利[1] 曾芳芳[1] 刘雄[1] 

机构地区：[1]南方医科大学南方医院耳鼻咽喉头颈外科,广东广州510515

出　　处：《南方医科大学学报》2015年第5期743-747,共5页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2013010015685)

摘　　要：目的：探讨miR-24在鼻咽癌患者血浆中的表达及临床意义。方法收集2007年12月~2011年6月在南方医院耳鼻喉科住院的初诊鼻咽癌患者血浆217例，以同期住院的慢性化脓性中耳炎或慢性鼻窦炎患者血浆73例作为对照，利用荧光定量PCR的方法进行miR-24表达的检测并结合临床资料分析其临床意义。结果血浆miR-24在鼻咽癌组的相对表达水平为对照组的4.808倍，差异具有显著统计学意义（P〈0.001）；血浆miR-24在不同T分期之间有统计学差异（P=0.007），miR-24与N分期呈负相关（P=0.028）；鼻咽癌病人血浆EBV-DNA与血浆miR-24呈负相关（P=0.048）；治疗后血浆miR-24含量较治疗前下降差异有显著统计学意义（P=0.001）。结论血浆miR-24可作为肿瘤分子标志物用于鼻咽癌早期诊断及预后分析。Objective To examine the expression level of miR-24 in the plasma of nasopharyngeal carcinoma （NPC） patients and investigate the clinical significance of miR-24 in NPC development. Methods Blood samples were from 217 NPC patients admitted in our Department between December, 2007 and June, 2011, with those from 73 patients with chronic purulent otitis media or chronic sinusitis as control. The follow-up data of all the patients were reviewed and the expression of miR-24 in the plasma was examined by qRT-PCR. The correlation of miR-24 expression with clinical staging of NPC was analyzed, and miR-24 levels before and after the treatment were compared. Results Compared with the control group, the NPC patients showed significantly up-regulated level of miR-24 in the plasma （P〈0.001）. Plasma miR-24 level differed significantly among patients with different T stages （P=0.007） and was negatively correlated with the N stages （P=0.028） and plasma EBV-DNA （P=0.048）. The expression levels of miR-24 were significantly reduced after treatment in the NPC patients and were significantly lowered in patients without relapse or metastasis （P=0.001）. Conclusion Plasma miR-24 may serve as a novel molecular biomarker for early diagnosis and prognosis of NPC.

关 键 词：血浆 miR-24 鼻咽癌 肿瘤分子标志物 

分 类 号：R739.63[医药卫生—肿瘤]