母婴分离大鼠血浆nesfatin-1与内脏敏感性的关系  

Effect of neonatal maternal separation on serum level of nesfatin-1 in rats

作　　者：周小平[1] 黄琳[1] 李学良[1] 林琳[1] 

机构地区：[1]南京医科大学第一附属医院消化内科,江苏省南京市210029

出　　处：《世界华人消化杂志》2015年第13期2104-2110,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81070308~~

摘　　要：目的:探讨新生期母婴分离对成年大鼠血浆nesfatin-1的影响及其与大鼠内脏敏感性的关系.方法:新生♂SD大鼠随机分为母婴分离组和正常组.母婴分离组大鼠自出生后第2-15天,每天上午8:00-11:00将幼鼠与母鼠分开,其余时间与母鼠共笼饲养,正常组幼鼠不做处理.第8周时,大鼠接受结直肠扩张(colorectal distension,CRD),检测腹壁撤退反射(abdominal withdrawal reflex,AWR)和腹外斜肌放电活动(electromyographic,EMG),ELISA法检测血浆nesfatin-1和糖皮质激素(glucocorticoid,GC)水平.结果:第8周时,母婴分离大鼠的体质量明显低于正常对照组.母婴分离大鼠血浆nesfatin-1、GC水平均明显高于正常大鼠,且血浆nesfatin-1水平与GC水平呈正相关.当CRD压力为40、60和80 mmHg时,母婴分离大鼠的AWR评分和EMG曲线下面积均明显高于正常对照.并且母婴分离大鼠的AWR评分和EMG曲线下面积均与血浆nesfatin-1水平呈正相关关系.结论:新生期母婴分离诱导成年大鼠的内脏高敏感性和血浆nesfatin-1水平的升高.内脏高敏感性可能与血浆nesfatin-1水平的升高有关.AIM： To explore the effect of neonatal maternal separation on the concentration of serum nesfatin-1 in rats. METHODS： Newborn rats were randomly male Sprague-Dawley divided into eithera maternal separation group or a control group. Rats in the maternal separation group received maternal separation from postnatal days 2-15. At the 8th postnatal week, the abdominal withdrawal reflex （AWR） and electromyographic （EMG） amplitude of the external oblique muscle in response to graded colorectal distension （CRD） were examined. The levels of serum nesfatin-1 and glucocorticoid were detected using ELISA.RESULTS： At the 8th postnatal week, the body weight of rats in the maternal separation group was significantly lower than that in the normal control group. The levels of serum nesfatin-1 and glucocorticoid in maternal separation group were statistically higher than those in the control group. The level of glucocorticoid was positively correlated with serum nesfatin-1 level. When the balloon pressure was set at 40, 60 and 80 mmHg, AWR score and EMG amplitude in the maternal separation group were significantly higher than those in the normal control group. AWR score and EMG amplitude in the maternal separation group were positively correlated with the level of serum nesfatin-1.CONCLUSION： Rats subjected to maternal separation showed a higher AWR score and EMG amplitude, as well as elevated levels of serum nesfatin-1. Visceral hypersensitivity induced by neonatal maternal separation may be associated with elevated level of serum nesfatin-1.

关 键 词：肠易激综合征 内脏高敏感 母婴分离 NESFATIN-1 

分 类 号：R574.4[医药卫生—消化系统]