胰岛素对大鼠局灶性脑缺血后脑水肿及水通道蛋白9的影响  

Effects of Insulin on Expression of Aquaporin-9 and Brain Edema after Focal Cerebral Ischemia in Rats

作　　者：李珊珊[1] 陈忠云[1] 李婧[1] 徐志伟[1] 杨旭[1] 

机构地区：[1]航天中心医院神经内科,北京100049

出　　处：《神经损伤与功能重建》2015年第3期191-193,212,共4页Neural Injury and Functional Reconstruction

基　　金：院级基因资助项目(No.YN201206)

摘　　要：目的:观察胰岛素对大脑中动脉栓塞(MCAO)大鼠模型脑水肿形成及水通道蛋白-9(AQP-9)表达的影响。方法:SD大鼠180只,随机分为假手术组、模型组、胰岛素组各30只,分别在栓塞后6、12、24、48、72 h观察6只。线栓法制备MCAO模型,假手术组不插入线栓,胰岛素组MCAO后立即给予腹腔注射胰岛素干预。各组进行神经功能评分,检测血脑屏障通透性、脑含水量和脑组织AQP-9 m RNA表达。结果:模型组和胰岛素组于缺血6 h后,脑含水量、血脑屏障通透性及AQP-9 m RNA表达均开始增加,于48 h达到高峰,之后逐渐下降,于72 h仍高于假手术组,有显著性差异(P<0.01);与模型组相比,胰岛素组中脑含水量、血脑屏障通透性及AQP-9 m RNA在各时间点均降低,差异有统计学意义(P<0.05);脑含水量、血脑屏障通透性与AQP-9m RNA表达呈显著正相关(r=0.905,P<0.01;r=0.923,P<0.01)。结论:胰岛素可能通过抑制AQP-9的表达减轻脑缺血后脑水肿的形成。Objective： To observe the effects of insulin on expression of Aquaporin-9（AQP-9） and brain edema after middle cerebral artery occlusion（MCAO） in rats. Methods： One hundred and eighty SD rats were randomly divided into groups of sham, MCAO and insulin treatment, with 30 rats in each group. Six rats in each group were observed at 6 h, 12 h, 24 h, 48 h, 72 h after MCAO. The MCAO models were estabilish by suture method, and the sham group without suture. The insulin treatment group was given insulin by intraperitoneal injection. The nerve function, blood-brain barrier permeability, brain water content and AQP-9 m RNA expression were evaluated.Results： The brain water content, blood-brain barrier permeability and AQP-9 m RNA expression of groups MCAO and insulin began to increase at 6 h and reached peak at 48 h and gradually decreased at 72 h, which were significantly higher than those in sham group（P〈0.05）. Compared with the MCAO group, the brain water content,blood-brain barrier permeability and AQP-9 m RNA expression in insulin treatment group were obviously decreased（P〈0.05）. There were a significantly passive correlation between AQP-9 m RNA expression and brain water contents, blood-brain barrier permeability（r=0.905, P〈0.01; r=0.923, P〈0.01）. Conclusion： Insulin can reduce brain edema through suppressing expression of AQP-9 in cerebral ischemia in rats.

关 键 词：脑缺血 脑水肿 胰岛素 AQUAPORIN-9 

分 类 号：R741[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]