不同氧浓度对创伤性轴索损伤合并低氧血症大鼠的影响  

Effect of Oxygen Concentration on Rats of Post-traumatic Axonal Injury with Hypoxia

作　　者：王杨[1] 温明哲 杨松 何裕超 朱坤灿[2] 庄步峰[2] 吴芳芳[2] 王洪财[4] 段志新[2] 马延斌[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,上海200127 [2]上海交通大学医学院附属第三人民医院神经外科,上海201900 [3]江苏省宿迁市第一人民医院神经外科,江苏宿迁223800 [4]浙江省宁波市医疗中心李惠利医院神经外科,浙江宁波315040

出　　处：《神经损伤与功能重建》2015年第3期198-200,共3页Neural Injury and Functional Reconstruction

基　　金：上海交通大学医学院科技基金项目(No.12XJ10029);上海市宝山区科委基金(No.10-E-2);浙江省宁波市自然科学基金(No.2011A610041)

摘　　要：目的:探讨不同氧浓度复苏对创伤性轴索损伤(TAI)合并低氧血症性二次脑损伤(SBI)大鼠模型的丙二醛(MDA)和超氧化物歧化酶(SOD)含量的影响。方法:48只SD大鼠,随机分为假手术组(A组)8只、损伤合并低氧血症组(B组)8只及损伤合并低氧后复苏组(C组)32只,根据不同氧浓度C组又分为21%、50%、75%、100%4个亚组。应用自制的TAI致伤装置制备模型,大鼠致伤后给予10%浓度氧吸入30 min制成低氧血症性SBI模型,之后给予不同氧浓度混合气体1 h,检测大鼠不同氧浓度通气复苏后24 h、1周时MDA、SOD变化情况。结果:与A组相比,B组及C组24 h时MDA含量明显升高,1周后恢复至正常水平;C组与B组比较,21%、50%亚组的MDA下降,差异有统计学意义(P<0.05);C组随氧浓度升高MDA值逐渐升高。B组及C组24 h时SOD含量明显下降,与A组比较差异有统计学意义(P<0.05),1周时仍低于正常水平;C组与B组比较,给予21%氧复苏时SOD值明显升高(P<0.05),给予50%、75%氧复苏时SOD差异无统计学意义(P>0.05),给予100%氧复苏时SOD值明显降低(P<0.05);C组随氧浓度升高SOD值逐渐降低。结论:适当的给氧治疗能改善和纠正脑组织缺氧,但随着给氧浓度的增加,MDA不断增高,SOD则不断降低。Objective： To explore the effects of oxygen concentrations on malondialdehyde（MDA） and superoxide dismutase（SOD） in secondary brain injury（SBI） rat model of post-traumatic axonal injury（TAI） with hypoxia.Methods： A total of 48 SD rats were divided into groups of sham（A, n=8）, TAI with hypoxia（B, n=8） and ventilating（C, n=32）. According to the different concentrations of oxygen, group C was divided into four subgroups（21%,50%, 75% and 100%）. TAI and systemic hypoxia were induced by an impact-acceleration ＆ rotation device and ventilating rats with 10% oxygen for 30 min, respectively. After that, the rats were given different concentrations of oxygen gas mixture for 1 hour. The MDA and SOD levels among those groups were systemically assessed and compared. Results： Compared to the group A, the MDA levels in the groups B and C were increased obviously,then returned to normal after one week. In the group C, the higher oxygen led to a higher MDA level. The SOD level dismutase in the groups B and C were decreased significantly, compared to that in the group A（P〈0.05）.Compared with that in the group B, the level of SOD in the subgroup ventilating with 21% oxygen in group C was significantly increased（P〈0.05）. In addition, while ventilating 100% oxygen, the SOD level decreased significantly（P〈0.05）. With the increase of ventilationg oxygen in group C, the level of SOD dramatically decreased. Conclusion： Appropriately ventilating oxygen can ameliorate hypoxemia in brain tissue. The level of MDA increases with ventilating higher concentration of oxygen, while the level of SOD decreases with increasing oxygen concentration.

关 键 词：创伤性轴索损伤 低氧血症 丙二醛 超氧化物歧化酶 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]