谷氨酰胺对实验性结肠炎BABL/C小鼠肠黏膜的修复作用  

Glutamine promotes intestinal mucosa repairing in experimental mouse colitis

作　　者：李力[1] 李弼民[2] 蔡敏[1] 程峰涛[1] 王斌[1] 黄玲[1] 

机构地区：[1]同济大学附属杨浦医院消化内科,上海200090 [2]南昌大学第一附属医院消化内科,江西南昌330000

出　　处：《同济大学学报（医学版）》2015年第2期11-15,共5页Journal of Tongji University(Medical Science)

基　　金：上海市卫生和计划生育委员会项目(20134424)

摘　　要：目的探讨谷氨酰胺(Gln)对BALB/C小鼠实验性结肠炎的治疗作用及可能的机制。方法将50只BALB/C小鼠分为正常对照组、模型对照组、5-ASA组、Gln组、Gln+5-ASA组。各实验性结肠炎组小鼠采用2,4,6-三硝基苯磺酸灌肠造模后,治疗组给予药物干预,观察疾病活动指数(disease activity index,DAI),取结肠组织行H-E染色病理组织学检查并积分,应用免疫组织化学技术检测小鼠结肠黏膜TNF-α、NF-κB、IL-10、IL-1β的表达。结果模型组小鼠DAI评分明显升高、结肠黏膜呈明显的炎症变化,表现为急性炎症,如溃疡形成、单核细胞浸润等。结肠黏膜TNF-α、NF-κB、IL-1β的表达增多、IL-10表达减少;谷氨酰胺能减轻小鼠大肠黏膜炎症,在一定程度上起到治疗小鼠实验性结肠炎的目的,其疗效与5-ASA相当;其中谷氨酰胺联合5-ASA治疗效果最佳,与正常对照组比较差异无统计学意义。结论谷氨酰胺可用于治疗BALB/C小鼠实验性结肠炎,其作用机制可能与促进肠黏膜细胞增殖、维护肠黏膜屏障、调节免疫功能、抑制NF-κB活化、降低TNF-α、IL-1β表达,升高IL-10的表达等有关,与5-ASA联用其治疗效果优于两者单独用药。Objective To evaluate the therapeutic effect of glutamine in treatment of experimental colitis in mice. Methods Fifty BALB / C mice were randomly divided into 5 groups： normal control group,model group,5-ASA group,glutamine group,and 5-ASA（ Acetyl-5-aminosalicylic acid） ＋glutamine group. The experimental colitis was induced with 2,4,6-Trinitrobenzenesulfonic acid（ TNBS） enema. The disease activity index（ DAI） was observed and the histopathological changes of intestinal mucosa were examined with H-E staining,the expression of TNF-a,NF-κB,IL-10 and IL-1βwas detected with immunohistochemical technique. Results The DAI score of model group increased significantly. Histopathological examination revealed acute inflammation,ulceration,mononuclear cell infiltration in intestinal mucosa in model mice. The expression of TNF-a,NF-κB,IL-β was increased,the expression of IL-10 was decreased. The glutamine can relieve intestine mucosa inflammation,to a certain extent,it can treat the experimental colitis mice. The curative effect is equal to 5-ASA. The therapy effect of combination of 5-ASA and glutamine group is best,and has no statistical difference compared with normal controls. Conclusion Glutamine can treat experimental colitis in mouse which may be related to promate mucosa cells growing,keep mucosa,relieve colon tissue injury in colitis by suppressing the activity of NF-κB,decrease TNF-α、IL-1β expression. The combination treatment of glutamine and 5-ASA has a better effect than either of individual treatment alone.

关 键 词：谷氨酰胺 结肠炎 细胞因子 免疫组化 小鼠 

分 类 号：R574.62[医药卫生—消化系统]