萃取耦合发酵工艺生产丁醇研究  

Liquid-liquid extraction fermentation for butanol production

作　　者：潘贺鹏 范新蕾[1] 罗玮[1] 李汉广[1] 余晓斌[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《工业微生物》2015年第3期1-7,共7页Industrial Microbiology

基　　金：国家自然科学基金(21176105)

摘　　要：萃取耦合发酵可有效减弱产物抑制和提高底物利用效率,本文就萃取耦合发酵生产丁醇工艺中的萃取剂的选择、萃取剂加入量、底物浓度等发酵条件进行了研究。结果表明:最佳萃取剂为大豆油生物柴油,油水比为3∶5,发酵过程无需搅拌,静置发酵为宜,在发酵之初加入萃取剂。分别以玉米和木薯为发酵底物,确定其最适底物浓度为100 g/L,以玉米为原料萃取耦合发酵中丁醇和总溶剂产量分别为18.17 g/L和29.31 g/L。以木薯为原料萃取耦合发酵生产丁醇及总溶剂产量比传统发酵分别提高了48.69%和51.80%。Extractive fermentation is an effective method to overcome the product inhibition in the fermentation process and increase the utilization rate of substrate. In this paper,the selection of extractive agent,ratio of extratant to water,substrate concentration and some other factors were investigated. The results showed that the best extraction solvent was soybean oil biodiesel and the ratio of extratant to water was 3∶ 5; the fermentation process was static fermentation without stirring and extratant was added to broth at the beginning of fermentation. The optimum concentration of substrate was identified as 100 g / L by comparing the corn and cassava raw materials. In the process of extractive fermentation with corn,the production of butanol and total solvent were 18. 17 g / L and 29. 31 g / L,respectively. In the process of extractive fermentation with cassava,the production of butanol and total solvent increased 48. 69% and 51. 80% than those of the traditional fermentation,respectively.

关 键 词：萃取耦合发酵 丁醇 生物柴油 

分 类 号：TQ923[轻工技术与工程—发酵工程]