氮离子注入诱变选育恩拉霉素高产菌株  

Breeding of high enramycin-producing strain from Streptomyces fungicidicus by N^+ ion beam irradiation

作　　者：蔡作新 郑传伟 田伟波 魏亚萍 艾咚咚 

机构地区：[1]齐鲁制药有限公司,山东济南250100 [2]山东齐发药业有限公司,山东平阴250400

出　　处：《工业微生物》2015年第3期58-61,共4页Industrial Microbiology

摘　　要：利用氮离子注入对链霉菌的诱变效应,筛选高产恩拉霉素的变异菌株。利用不同剂量的氮离子对杀真菌放线菌S.fungicidicus NL629-3菌株进行诱变处理,研究低能氮离子注入对其存活率及产恩拉霉素能力的影响。低能氮离子注入剂量在60×1013ions/cm2时对链霉菌的诱变效应显著,试验得到了5株恩拉霉素产量较高的突变菌株,其中N3-643菌株经连续传代4次,遗传稳定性较好,其摇瓶发酵水平较对照提高了41%,放大发酵生产后平均发酵水平提高25.8%。离子注入诱变是获得高产恩拉霉素突变菌株的有效方法。The mutagenic effects of nitrogen ion（ N＋） beam irradiation on Streptomyces fungicidicus were used to obtain high-yielding strain for producing enramycin. Different doses of N＋implantation were used to accomplish the mutation of Streptomyces fungicidious NL629-3 strain. Then,the effects of low energy N＋implantation on the survival rate and enramycin-producing ability of the mutant stains were investigated. The results indicated that low energy N＋implantation of 60× 10^13 ions / cm^2 could efficiently improve the positive mutation rate of Streptomyces fungicidious and 5 mutant strains with higher yielding enramycin were obtained. The mutant strain N3-643 had better inheritance and fermentation stability after four continuous generations. The yield of enramycin were increased by 41% in shake-flask culture and 25. 8% on largescale production if compared with those of the control. N＋ion beam irradiation was an effective method to obtain highyielding enramycin strain.

关 键 词：链霉菌 氮离子注入 恩拉霉素 诱变育种 

分 类 号：TQ927[轻工技术与工程—发酵工程]