重楼皂苷Ⅱ对A375人黑素瘤细胞增殖和凋亡的影响  

The effect of Polyphyllin Ⅱ on the proliferation and apoptosis of A375 human melanoma cell

作　　者：姜福琼[1] 邓丹琪[1] 王剑松[1] 刘馨[1] 张佩莲[1] 周晓鸿[1] 

机构地区：[1]昆明医科大学第二附属医院,云南昆明650101

出　　处：《皮肤病与性病》2015年第3期125-128,共4页Dermatology and Venereology

基　　金：云南省教育厅基金(项目编号:2013C248)

摘　　要：目的研究重楼提取物单体重楼皂苷Ⅱ在体外对A375人黑素瘤细胞增殖和凋亡的影响。方法体外培养A375人黑素瘤细胞株,采用MTT法检测重楼皂苷Ⅱ对细胞增殖的影响,计算IC50值;透射电镜观察细胞超微结构的变化;采用流式细胞术检测重楼皂苷Ⅱ干预后A375人黑素瘤细胞凋亡的情况。结果重楼皂苷Ⅱ对A375人黑素瘤细胞的增殖有明显的抑制作用,24h的IC50值为12.04μmol/L。重楼皂苷Ⅱ对人A375细胞生长的抑制作用表现出时间和浓度依赖性。另外重楼皂苷Ⅱ引起A375黑素瘤细胞的凋亡率显著增加。结论重楼皂苷Ⅱ对A375人黑素瘤有潜在的抗癌作用,可能是通过诱导黑素瘤细胞凋亡而发挥作用。Objective To investigate the potential effect of polyphyllin Ⅱ （PP Ⅱ ） on the proliferation and apoptosis of A375 human melanoma cell. Methods A375 Human melanoma cells were cultured with or without PP Ⅱ. The proliferation of cells was measured by MTT assay. The changes of cell morphology were observed by transmission electron microscopy. The apoptosis and cell cycle were measured by flow cytometry. Results The results have shown that the proliferation of A375 human melanoma cells were significantly inhibited by PP Ⅱ. The 50% inhibitory concentration （IC50） of PP Ⅱ were 12.04μmol/L at 24 h. Conclusion The inhibition of PP Ⅱ to melanoma cell was in a dose and time-dependent manner. After being treated with PP Ⅱ , the apoptotic rate of cells was remarkably higher than that of the control. Polyphyllin Ⅱ potentially has anticancer effects on A375 human melanoma cells in vitro, which result from increased cell apoptosis.

关 键 词：黑素瘤 重楼皂苷Ⅱ 中药 

分 类 号：R739.5[医药卫生—肿瘤]