湖北产葛根药材及提取物指纹图谱分析  

Study on Fingerprints of Radix Puerariae and its Extract in Hubei Province

作　　者：刘慧敏[1] 徐惠芳[1] 邵贝贝 

机构地区：[1]武汉市中医医院药学部,湖北武汉430010 [2]武汉联合药业,湖北武汉430011

出　　处：《亚太传统医药》2015年第11期25-29,共5页Asia-Pacific Traditional Medicine

摘　　要：目的：建立湖北产葛根药材及提取物的指纹图谱检测方法,为确保葛根药材、提取物及其制剂的内在质量提供依据.方法：采用TIANHE Kormasil C18柱;流动相A：甲醇,流动相B：1％醋酸溶液;流速1.0mL＆#183;min-1;检测波长为250nm;柱温为35℃.梯度洗脱程序：0～10min 22％A,10～40min 22％～45％A,40～45min 45％A.对湖北省十堰竹溪、恩施州和黄冈罗田三个产地的葛根药材及提取物进行指纹图谱研究.结果：湖北省三个产地葛根药材及提取物的指纹图谱基本一致.结论：湖北三个产地的葛根药材质量差异不大.葛根药材、提取物相关性良好,各产地指纹图谱基本一致,制备工艺稳定可行.Objective： To establish the fingerprints of Radix Puerariae and its extract in Hubei Province for the quality control.Methods ： The fingerprints of Radix Puerariae and its extract were established by HP LC. The chromatographic separation was per-fermed on a TIANHE Kormasil C18 column（4.6 mmX 150 mm, 5m）. The mobile phase consisted of methanol and 1 % acetic acidwith gradient elution. The f low-rate was 1.0mL · min-1 , the column temperature was at 35℃and the UV absorbance detection wasat 250nm. Results： Under the selected chromate graphic conditions, a good fingerprint of Radix Puerariae and its extract were ob-tained. The fingerprint of Radix Puerariae from Zhuxi Shiyan, Enshi and Luotian Huanggang were basically identical. Conclusion：The quality of Radix Puerariae and its extract can be controlled effectively by HPLC-UV fingerprint. The Preparation process of theextract from Radix Puerariae is stable and feasible.

关 键 词：葛根药材 葛根提取物 指纹图谱 质量控制 

分 类 号：R284[医药卫生—中药学]