茶多酚对胶原诱导性关节炎大鼠足爪组织MMP-2蛋白表达水平的影响  

Effect of Tea Polyphenols on paw MMP-2 protein expression in collagen-induced arthritis rats

作　　者：陈永顺[1] 李静[1] 蒋建平[1] 梁建梅[1] 贾晓栋[2] 

机构地区：[1]商丘医学高等专科学校,河南商丘476100 [2]广西医科大学药学院,广西南宁530021

出　　处：《安徽医药》2015年第6期1059-1062,共4页Anhui Medical and Pharmaceutical Journal

基　　金：河南省教育厅科学技术研究重点项目(No 13B310204)

摘　　要：目的探讨茶多酚对Ⅱ型胶原乳化剂诱导关节炎大鼠的治疗作用及机制。方法 SD大鼠随机分为正常对照组、模型组、地塞米松对照组(2 mg·kg-1·d-1)和茶多酚治疗组(200 mg·kg-1·d-1),采用Ⅱ型胶原弗氏完全佐剂法(collagen-induced arthritis,CIA)建立类风湿性关节炎大鼠模型。记录大鼠足趾体积的变化,酶联免疫法(ELISA)检测大鼠血清IL-1β、PGE2水平,免疫组化法检测大鼠足爪组织基质金属蛋白酶2(MMP-2)蛋白水平的表达。结果与模型组比较,茶多酚治疗组大鼠踝关节肿胀明显减轻(P<0.05),大鼠血清IL-Iβ、PEG2水平明显降低(P<0.05),足爪组织细胞中MMP-2蛋白表达明显下降(P<0.05)。结论茶多酚对CIA大鼠关节炎有抑制作用,其机制可能与抑制炎症介质IL-1β、PEG2,下调MMP-2蛋白表达有关。Objective To investigate the effect of Tea polyphenols on collagen-induced arthritis（CIA）rats,and to explore its therapeutic mechanism.Methods SD rats were randomized into four groups：normal control group,model group,dexamethasone group （2 mg · kg^-1 ·d^-1 ）and tea polyphenols group（200 mg·kg^-1 ·d^-1 ）.The rats were given pedal intradermal injection of collagen and Com-plete Freund＇s Adjuvant to induce arthritis.We recorded the changes of rats in toe volume .The serum content of IL-1βand PGE2 were assayed by ELISA,and the expression of MMP-2 on rats paw was detected by immunohistochemical staining.Results As compared with the model group,the ankle joint swelling degree was relieved in tea polyphenols group （P〈0.05 ）,the serum levels of IL-1β＆amp;PEG2 were significantly decreased（P〈0.05）,and the expression of MMP-2 protein on paw also decreased（P〈0.05）.Conclusions Tea polyphenols has an obvious inhibition on CIA rats,and its mechanism may be related to the reduction of IL-1 β＆amp;PEG2 ,and down-regulation of MMP-2.

关 键 词：茶多酚 胶原诱导性关节炎 基质金属蛋白酶2 MMP-2 

分 类 号：R285.5[医药卫生—中药学]