胡芦巴胶降解工艺研究  

Study on degradation process of fenugreek gum

作　　者：贺少龙[1] 冯军[1] 洪彤彤[1] 隋宏[1,2] 王文苹[1,2,3] 

机构地区：[1]宁夏医科大学药学院,宁夏银川750001 [2]宁夏回药现代化工程技术研究中心,宁夏银川750004 [3]回医药现代化省部共建教育部重点实验室,宁夏银川750004

出　　处：《药学研究》2015年第6期311-314,334,共5页Journal of Pharmaceutical Research

基　　金：国家自然科学基金项目(No.30020105;No.81160521)

摘　　要：目的优选胡芦巴胶的降解方法及工艺,获得质量稳定可控的低分子量多糖。方法以总糖和还原糖含量以及黏度为评价指标,比较酸水解、超声辅助、酶解等工艺,优选最佳条件。并依据紫外、红外及差示扫描热图谱对产物进行初步评价。结果不同降解工艺所得还原糖得率分别为酶解52.41%、超声辅助42.19%、酸解24.59%;以纤维素酶用量10 U·mg-1、50℃处理150 min时,降解效果最佳。重复实验表明该工艺稳定可控,紫外、红外及DSC图谱无明显差异,峰位基本不变。结论酶法降解胡芦巴制备低分子量多糖,方法简便、高效、易于控制。Objective To optimize the degradation process of fenugreek gum and to obtain low- molecular- weight polysaccharide with stable and controllable quality. Methods Taking total sugar,reducing sugar content and viscosity parameter as evaluating index,degradation methods of traditional acid hydrolysis,ultrasound- aided acid hydrolysis and enzymatic degradation were compared and the process conditions were further optimized. The fenugreek gum and its degradation products were characterized by UV,IR and DSC spectrometry respectively. Results The reducing sugar content of different degradation methods were 52. 41% for enzymatic degradation,42. 19% for ultrasound- aided acid hydrolysis and 24. 59%for traditional acid hydrolysis. The optimum process was as follows: Fenugreek gum solution was treated with 10 U cellulose at 50 ℃ for 150 min. Repeated experiments showed that the degradation process was stable and controllable. No significant differences were found in the UV,IR or DSC spectrums between fenugreek gum and its degradation products. Conclusion Enzymatic degradation of fenugreek gum turned was an efficient,simple and controllable method.

关 键 词：胡芦巴胶 酶法降解 黏均分子量 低分子量多糖 

分 类 号：R284.2[医药卫生—中药学]