高盐低温胁迫下水稻叶细胞ROS清除系统的相关基因表达  

Expression Profiling of Rice ROS Scavenging System Related Genes under Salt or Low Temperature Stress

作　　者：赵静[1] 梁建生 吴雪玲[1] 刘晓琴[1] 栗会召 朱素琴[1] 

机构地区：[1]南通大学生命科学学院,江苏南通226007 [2]南方科技大学,广东深圳518055

出　　处：《西北植物学报》2015年第5期872-883,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30270794);江苏省南通市科技计划项目(HL2013030);大学生创新训练计划项目(201310304021Z)

摘　　要：采用Affymetrix水稻表达谱芯片,分析高盐和低温胁迫下水稻叶细胞内ROS清除系统的相关基因表达状况,探讨在响应非生物胁迫过程中水稻植株体内抗氧化防卫体系的积极作用。结果表明:(1)水稻叶细胞内ROS清除系统涉及187个基因和/或EST,由抗氧化的非酶类物质如抗坏血酸、谷胱甘肽、生育酚等和抗氧化酶如超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)等组成。(2)在低温逆境下,籼稻(i-93-11)叶细胞表达上调基因(2倍以上,下同)有5个,下调(0.5倍以下,下同)基因有2个;粳稻(j-NJ-1)叶细胞表达上调基因5个,下调基因6个。(3)在高盐胁迫下,籼稻(i-93-11)叶细胞表达上调基因有31个、下调基因13个;粳稻(j-NJ-1)叶细胞表达上调基因27个,下调基因25个。(4)高盐和低温胁迫下水稻叶细胞ROS清除系统相关基因的表达在籼粳稻间存在较大差异,并根据水稻基因表达谱芯片数据构建了水稻响应高盐和低温胁迫ROS清除网络图。To explore a positive action of the antioxidant defense system in rice plant response to amonc stress,we used the Affymetrix Rice GeneChip to deduce the expression profiling of rice ROS scavenging system related genes under salt-stress or low temperature-stress treatment. The results showed that. （1） Rice ROS Scavenging System involves 187 genes and/or ESTs that were composed of antioxidants,such as ascorbate,glutathione, tocopherols （Vitamin E）, and antioxidant enzymes, such as superoxide dismutase （SOD） ,ascorbate peroxidase （APX）, catalase （CAT） and so on. （2）In comparison with the control, there were 5 up-regulation genes （fold change≥2.0, P〈0.05,the same as below） and 2 down-regulation genes （fold change≤0.5, P〈0.05, the same as below） in Indica cultivar i-93-11 under low temperature stress; otherwise,there were 5 up-regulated genes and 6 down-regulated genes in Japonica cultivar j-NJ-1 under low temperature stress. （3）There were 31 up-regulated genes and 13 down-regulated genes in i-93-11,27 up-regulated genes and 25 down-regulated genes in j-NJ-1 under high salinity. （4）There was a big differ ence between the expression profiling of ROS scavenging system related genes under salt-stress or low temperature-stress treatment in Indica and Japonica rice. Furthermore,a ROS scavenging network planning in rice responses to high salt and tow temperature stress was constructed according to the Affymetrix Rice GeneChip.

关 键 词：ROS 表达谱 高盐 低温 水稻 

分 类 号：Q789[生物学—分子生物学] Q945.78