利用生物酶降解烟草果胶的研究  

Research on degradation of pectin using enzyme treatment in flue-cured tobacco

作　　者：朱晓兰[1] 刘百战[2] 王维妙[2] 高芸[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心,安徽合肥230052 [2]上海烟草集团有限责任公司烟草行业卷烟烟气重点实验室,上海200082

出　　处：《湖南农业大学学报（自然科学版）》2015年第3期257-262,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家烟草专卖局烟草行业卷烟烟气重点实验室开放基金项目(SZBCW201300708);川渝中烟工业有限责任公司项目(JL/CYZYG SJ003–04)

摘　　要：研究了果胶甲酯酶、果胶裂解酶、多聚半乳糖醛酸酶、纤维素复合酶处理烟叶,细胞壁物质降解后,烟叶中果胶的含量与结构以及中性糖比例的变化。结果显示:4种生物酶可以有效降低烟叶中细胞壁类物质的含量,最高降幅可达17.8%;果胶甲酯酶和果胶裂解酶处理后的烟样,果胶的甲酯度显著降低,多聚半乳糖醛酸酶处理后果胶甲酯度略有增大;酶处理后果胶中性糖的含量均比对照样增加,但不同种类酶处理的中性糖比例有区别。果胶裂解酶和纤维素酶处理后,烟叶中性香气物质总量(不含新植二烯)分别提高了24.4%和21.9%。In this work, pectinesterase, pectinlyase, polygalacturonase and cellulose-preparation enzyme were used to decompose cell wall substances of flue-cured tobacco, and the contents and structure of the pectin in tobacco and the changes of the ratios of neutral sugar in pectin were investigated. The result showed that the 4 enzymes could decompose cell wall substances in tobacco effectively, and cell wall substances were decreased with the highest decrease of 17.8%. The degree of methylation in pectin was decreased evidently in tobacco treated with pectinesterase and pectinlyase while increased slightly in tobacco treated with polygalacturonase. The contents of neutral sugar in pectin increased in tobacco treated with the 4 enzymes, but there were much difference in the ratios of neutral sugar with different enzymes. The total contents of flavor components（without neophytadiene） in tobacco treated with pectinlyase and polygalacturonase were increased by 24.4% and 21.9%, respectively.

关 键 词：烟叶 果胶 结构 生物酶 降解 

分 类 号：S572.01[农业科学—烟草工业]