虎杖提取物白藜芦醇对放射性肺损伤防护作用机制研究  

Mechanism research of the protective effects of resveratrol extracted from Polygonum cuspidatum on radiation- induced pulmonary injury

作　　者：阎皓[1] 张恒[1] 辛绍斌[2] 王辉[1] 

机构地区：[1]天津市人民医院肿瘤科,天津300121 [2]天津市人民医院重症医学科,天津300121

出　　处：《河北中医》2015年第5期721-724,727,共5页Hebei Journal of Traditional Chinese Medicine

基　　金：天津市中医药管理局中医中西医结合科研课题(编号:13131)

摘　　要：目的观察虎杖提取物白藜芦醇(Rev)对放射诱导小鼠肺损伤的保护作用及其防护机制。方法将30只C57BL/6雄性小鼠实验小鼠随机分为对照组、照射组和照射给药组,每组各10只。照射组和照射给药组小鼠用1%戊巴比妥钠(30 mg/kg)腹腔注射麻醉后固定于照射板架上,每日接受1次137Cs源γ射线胸部照射,共照射5次。对照组小鼠接受伪照射。照射给药组照射期间及照射后2周予Rev 40 mg/kg每日灌胃给药,对照组和照射组予等量4.8%乙醇载体灌胃。末次照射后15 d,全身麻醉,处死小鼠。观察3组小鼠肺组织病理改变,检测丙二醇(MDA)含量,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX1)活性和基因表达水平。结果与照射组比较,照射给药组在小鼠肺泡炎水平明显改善,肺组织MDA含量显著减少,SOD及GPX1酶活性升高,SOD2及GPX1基因表达水平升高(P<0.05)。结论虎杖提取物Rev可以提高放射性肺损伤小鼠肺组织的氧化应激水平,改善肺组织炎症。Objective To observe the protective effects and protective mechanism of resveratrol （Rev） ex-tracted from Polygonum cuspidatum on radiation induced lung injury in mice .Methods 30 C57BL/6 male laborato-ry mice were randomly divided into control group , irradiation group and irradiation drug administration group , 10 mice of each group .Mice in irradiation group and irradiation drug administration group received peritoneal injection of 1%pentobarbital sodium （30 mg/kg）, and then were fastened to irradiation grillage .Mice in irradiation group and irra-diation drug administration group received thoracic irradiation of 137 Cs source gamma rays once daily , 5 times totally. Mice in control group received pseudo irradiation .Mice in irradiation drug administration group received daily intra-gastric administration of Rev （40 mg/kg） during irradiation and 2 weeks after irradiation.Mice in other two groups received daily intragastric administration of carrier of 4.8%ethanol at the same dose .Mice in all groups were sacri-ficed 15 days after the final irradiation .The pathological changes of lung tissue , the content of propanediol （ MDA） , the enzyme activities of Chaoyang tiger dismutase （ SOD） and glutathione peroxidase （ GPX1 ） and the expression of gene in mice were observed in 3 groups .Results The level of pulmonary alveolitis in irradiation drug administration group had obviously improved as compared with that in irradiation group , and the content of MDA in lung tissue was decreased , the activities of SOD and GPX 1 were increased , the gene expressions of SOD 2 and GPX1 were increased （ P 〈0.05）.Conclusion Resveratrol extracted from Polygonum cuspidatum can increase the level of oxidative stress in lung tissue of irradiating mice , and improve the infection reaction of lung tissue .

关 键 词：肺 损伤 疾病模型 动物 虎杖 化学 藜芦生物碱类 药理学 

分 类 号：R-332[医药卫生] R282.71