葡萄糖调节蛋白78通过调节上皮-间叶转化促进乳腺癌MCF-7的侵袭转移  

Glucose regulated protein 78 promoted the invasion and metastasis of breast cancer MCF-7 by regulating epithelial-mesenchymal transformation

作　　者：于健 张秀秀 于蓝青 孟令新[3] 

机构地区：[1]日照市东港区人民医院肿瘤科,山东省276800 [2]宿迁市疾病预防控制中心质管科,江苏省223800 [3]济宁医学院附属日照市人民医院肿瘤科,山东省276826

出　　处：《中华临床医师杂志（电子版）》2015年第9期93-95,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：济宁医学院面上项目(JY2013KY061)

摘　　要：目的探讨葡萄糖调节蛋白78(GRP78)对乳腺癌细胞MCF-7侵袭转移能力的影响和可能的分子机制。方法 p EGFP-N1-GRP78质粒转染至MCF-7细胞,通过筛选克隆获得GRP78高表达MCF-7细胞株(MCF-7-GRP78),同时将p EGFP-N1空载体转染细胞(MCF-7-空)作对照组,未处理细胞(MCF-7)作为空白对照。荧光显微镜观察绿色荧光,确定转染效果。通过细胞划痕实验检测三组细胞的迁徙能力,Western blot法检测三组细胞中GRP78、E-钙黏素、N-钙黏素的表达情况。结果 (1)荧光显微镜观察细胞转染效果均很好。Western blot结果显示,与MCF-7细胞相比,MCF-7-GRP78细胞中GRP78含量明显增加(P<0.05),而MCF-7-空细胞中GRP78的表达含量与之比较差异无统计学意义(P>0.05)。(2)细胞划痕实验显示,与MCF-7细胞相比,MCF-7-GRP78细胞的愈合能力明显增强,而MCF-7-空细胞与之差别不大。(3)Western blot结果显示:与MCF-7细胞相比,MCF-7-GRP78中N-钙黏素表达量升高(P<0.05),E-钙黏素表达量下降(P<0.05),而MCF-7-空细胞中E-钙黏素、N-钙黏素的表达含量与之差异无统计学意义(P>0.05)。结论 GRP78是一个促癌基因,GRP78可能通过上皮-间叶转化促进乳腺癌细胞的侵袭转移。Objective To study the effect and mechanisms of glucose regulated protein 78（GRP78） on the invasion and metastasis of breast cancer cell MCF-7. Methods MCF-7 cells which was transfected by pEGFP-GRP78 full length eukaryotic can stably overexpression GRP78 was called MCF-7-GRP78, while the pEGFP empty vector transfected cells （MCF-7-empty） as control, untreated cells （MCF-7） as a blank control. The expression of green fluorescent was observed by fluorescent microscopy to ensure the transfection efficiency. Wound Healing assay was used to detect the migration ability of the three group cells, the expression of GRP78, E-cadherin and N-cadherin were detected by Western blot. Results Transfection efficiency were high which was detected by the fluorescence microscope. Western blot results showed that, the expression of GRP78 in MCF-7-GRP78 were significantly increased （P〈0.05）, while the difference between MCF-7-empty cells and MCF-7 had no statistical significance （P〉0.05） compared with MCF-7 cells. Compared with MCF-7 cells, the healing ability of MCF-7-GRP78 cells was significantly enhanced, while MCF-7-empty cell had no statistically significance using the Wound Healing assay. Compared with MCF-7 cells, N-cadherin expression levels was increased and E-cadherin expression level was decreased in MCF-7-GRP78 （P〈0.05）, while the expression of E-cadherin and N-cadherin has no significant difference in MCF-7-empty （P〉0.05） by Western blot methods. Conclusion GRP78 is a cancer-promoting gene which can promote the invasive ability of breast cancer through epithelial-mesenchymal transition.

关 键 词：乳腺肿瘤 GRP78 E-钙黏素 N-钙黏素 侵袭转移 

分 类 号：R737.9[医药卫生—肿瘤]