硫化氢对蛛网膜下腔出血大鼠额顶叶脑区神经损伤的保护作用  

Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage

作　　者：刘艳梅[1] 朱倩[1] 令狐艳[2] 王建强[3] 刘珺[1] 李文杰[1] 王旻晨[1] 吴开云[1] 

机构地区：[1]苏州大学医学部解剖学教研室,江苏苏州215123 [2]贵阳医学院人体解剖学教研室,贵阳550004 [3]江苏大学附属金坛医院重症监护室,江苏金坛213200

出　　处：《解剖学报》2015年第3期297-303,共7页Acta Anatomica Sinica

基　　金：苏州医学发展基金资助项目(EE134519);江苏高等学校优势学科建设工程资助项目

摘　　要：目的探讨外源性硫化氢对蛛网膜下腔出血(SAH)大鼠早期脑损伤的保护作用及可能机制。方法30只清洁级SD大鼠随机分成3组:对照组、SAH模型组、硫化氢作用组,每组各10只。后两组大鼠应用大脑中动脉穿刺法制作SAH模型,硫化氢组于模型制作成功后立即腹腔注射100mg/kg硫氢化钠。各组大鼠24h后进行神经功能评分,取额顶叶脑组织,用免疫荧光和Western blotting等方法检测额顶叶脑区神经细胞GFAP、S-100b、B淋巴细胞瘤-2基因(Bcl-2)、Caspase-3、c-Fos、基质金属蛋白酶-9(MMP-9)蛋白表达的变化。结果硫化氢组与SAH组相比神经功能评分有明显改善(P<0.01)。硫化氢组神经评分得3分以上的只有2只动物,而SAH组有8只。免疫荧光结果显示,SAH组GFAP、S-100b蛋白表达较对照组明显增高(P<0.05);硫化氢作用后两者表达较SAH组明显下降(P<0.05)。SAH组Bcl-2、Caspase-3均有表达,硫化氢作用后Bcl-2表达明显增加,而Caspase-3则明显下降(P<0.05)。Western blotting结果进一步显示,SAH组c-Fos、MMP-9表达较对照组均明显增高(P<0.05);硫化氢作用后两者表达则明显下降(P<0.05)。结论硫化氢对SAH后脑损伤有明显保护作用,其机制可能与改善胶质细胞的功能及与凋亡机制有关。Objective To explore the effect of hydrogen sulfide （H2S） on the brain injury in rats with subarachnoid hemorrhage （SAH）. Methods Thirty experimental rats Were randomly divided into control group （n = 10） , SAH model group （n = 10） and NariS （ 100 mg/kg） group （n = 10）. The experimental rats of the H2 S group were injected with NariS 100 mg/kg body weight in intraperitoneal and after surviving for 24 hours to do the neurological assessment and took the whole brain. The expression changes of GFAP, S-100b, B-cell lymphoma-2 （Bel-2）, e-Fos, Caspase-3, matrixmetalloproteinase-9 （MMP-9） were detected with immunofluoreseenee and Western blotting method. Results Neurological assessment showed that severe neurological symptoms were significantly relieved in hydrogen sulfide group. GFAP and S-100b had a high expression in SAH rats group and were significantly decreased in H2S pretreatment group. The expression of Bel-2 and Caspase-3 in SAH group were high than that in the control group but the expression of Bcl-2 was increased in H2S pretreatment group than SAH group, and Caspase-3 was decreased in H：S pretreatment group. Western blotting analysis showed that the expression of c-Fos and MMP-9 were increased in SAH group and was significantly decreased in H2S pretreatment group. Conclusion The hydrogen sulfide has an significant protective effect onbrain injury after SAH and its mechanism ,nay be improved the function of glial cells and apoptotic pathways.

关 键 词：蛛网膜下腔出血 硫化氢 S-100B B淋巴细胞瘤-2基因 C-FOS 基质金属蛋白酶-9 免疫荧光 大鼠 

分 类 号：R743.9[医药卫生—神经病学与精神病学]