二氢杨梅素对肺癌A549细胞凋亡的作用  

Effects of dihydromyricetin on apoptosis of lung adenocarcinoma A549 cells

作　　者：孙大永[1] 左彦珍[2] 梁鸿鹄[1] 宋冀[1] 马春虎[2] 孙昊阳 

机构地区：[1]承德市中心医院放化疗中心,河北承德067000 [2]承德医学院药理学教研室,河北承德067000 [3]承德颈复康药业,河北承德067000

出　　处：《解剖学报》2015年第3期359-362,共4页Acta Anatomica Sinica

基　　金：河北省高等学校科学技术研究资助项目(QN2014036;QN20131010);河北省卫生厅重点课题资助项目(ZL20140247);承德市科学技术研究资助项目(20142015);承德医学院自然科学资助项目(201407)

摘　　要：目的探讨二氢杨梅素(DMY)对肺癌A549细胞的诱导凋亡作用。方法以肺癌A549细胞为研究对象,采用MTT法研究DMY对A549细胞的抗肿瘤作用,并进一步通过流式细胞术检测DMY对A549细胞的凋亡作用,免疫印迹法(Western blotting)检测促凋亡因子Bax和抗凋亡因子Bcl-2表达水平,进一步探讨其机制。结果DMY浓度越大,对A549细胞的抑制作用越明显,48h的IC50为64.45g/L;流式细胞术显示,随着DMY浓度的增加A549细胞凋亡也逐渐增加;Western blotting结果显示,DMY可诱导A549细胞中凋亡因子Bax蛋白表达增加,同时抗凋亡因子Bcl-2蛋白表达减少,尤其高浓度组改变明显。结论 DMY可以在体外诱导A549细胞凋亡。Objective To study the effect of dihydromyricetin on apoptosis of hunman non-small cell lung cancer A549 cells, and to explore new drugs for lung cancer. Methods A549 cells were treated with various concentrations of dihydromyricetin for 48hours. MTT assay was used to determined the proliferation ability of A549 cells, flow cytometry was used to detect the influence of dihydromyricetin on the cell apoptosis of A549 cells, Western blotting analysis was used to evaluate the levels of Bax, Bcl-2 expressions. Results MTT assay results showed a significant inhibition in A549 cells with a dose-dependent manner, when compared with control cells, the inhibitory concentration for 50% （IC50）was 64. 45mg/L at 48 hours; Flow cytometry showed that dihydromyricetin induced apoptosis of A549 cells in a dose-dependent manner as well. Further analysis by Western blotting showed that the expression of Bax was increased, while the expression of Bcl-2 was reduced when A549 cells was incubated for 48 hours with dihydromyricetin. Conclusion Dihydromyricetin plays a role of anti-tumor through inhibiting A549 cells proliferation and inducing apoptosis.

关 键 词：二氢杨梅素 A549细胞 肺癌 免疫印迹法 流式细胞术 人 

分 类 号：R734.2[医药卫生—肿瘤]