耐药基因MDR1(P-gp)和ABCG2在Hep-2细胞耐药中的作用研究  

The effect of MDR1(P-gp)and ABCG2 on the drug resistance in Hep 2 cells

作　　者：孙臻峰[1] 沈斌[1] 张佳[1] 苏甜甜[1] 董频[1] 

机构地区：[1]上海市第一人民医院耳鼻咽喉头颈外科,上海200080

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第11期1016-1019,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨耐药基因MDR1(P-gp)和ABCG2在Hep-2细胞耐药中的作用。方法:应用流式细胞仪检测MDR1和ABCG2抑制剂对于Hep-2喉癌细胞株积聚和外排抗肿瘤药物功能的影响,以及MDR1和ABCG2抑制剂对于Hep-2喉癌细胞株增敏作用的影响。结果:ABCG2抑制剂FTC在Hep-2细胞对Mitoxantrone吸收、外排检测实验中与对照组比较,差异有统计学意义;P-gp在该实验中与对照组差异无统计学意义;当抗肿瘤药物为Mitoxantrone及Cisplatin时,P-gp及ABCG2拮抗剂的加入对Hep-2细胞活力检测与对照组比较,差异无统计学意义;当抗肿瘤药为Doxorubicin时,FTC、P-gp单独作用对Hep-2细胞活力影响与对照组比较差异无统计学意义;而当FTC+P-gp时,对Hep-2细胞活力影响与对照组比较差异有统计学意义(P<0.05);当抗肿瘤药为5-FU时,P-gp对Hep-2细胞活力影响相比对照组差异有统计学意义(P<0.05),而FTC对该药耐药几乎无任何影响,FTC+P-gp也无效;当抗肿瘤药为Paclitaxel时,P-gp对Hep-2细胞活力影响相比对照组差异有统计学意义,FTC+P-gp对Hep-2细胞活力影响相比对照组差异有统计学意义(P<0.05)。结论:ABCG2可能主要通过影响化疗药物在细胞中的积聚与外排导致细胞耐药。P-gp产生耐药的方式可能有其他途径。P-gp和ABCG2在不同的化疗药物耐药作用中扮演不同的角色。Objective：To study the effect of MDR1 （P-gp） and ABCG2 on the drug resistance in Hep 2 cells. Method：Flow cytometry was used to detect the variations of the antitumor drugs accumulation and discharging, and activity variations when MDR1 and ABCG2 inhibitors were used in Hep-2. Result： The accumulation and dis- charging of mitoxantrone was significantly higher than the control group when ABCG2 inhibitor FTC was used in Hep-2 （P〈0.05）. In contrast, P-gp did not appear similar case; To the mitoxantrone and cisplatin, there was no statistical correlation about activity of Hep-2 between P-gp or ABCG2 antagonist and the control; To the doxorubicin, combining FTC and P-gp, the activity of Hep-2 was higher than the control and difference was significant （P〈0.05）, In contrast, FTC and P-gp did not appear similar case when use/ alone; To the 5-FU, when PGP used, the activity of Hep-2 was higher than that in the control and difference was significant （P〈0.05）, In con- trast, FTC and FTC＋P-gp did not appear similar case; To the paelitaxel, wl-en P-gp or FTC＋P-gp used, the activity of Hep-2 was higher than that in the control and difference was significant（P〈0.05）. Conclusion： ABCG2 may lead to drug resistance mainly by changing the ability of cell in accumulating and discharging chemotherapy drugs. P-gp has other way. P-gp and ABCG2 play different roles in different drug resistance.

关 键 词：耐药 喉肿瘤 化疗药物 

分 类 号：R739.65[医药卫生—肿瘤]