不同荞麦品种落粒基因的筛选  

Screening of Seed-holding Gene in Different Buckwheat Varieties

作　　者：李雪[1] 黄志强[1] 姜君[1] 陈庆富[1] 

机构地区：[1]贵州师范大学,贵州贵阳550001

出　　处：《贵州农业科学》2015年第5期19-23,共5页Guizhou Agricultural Sciences

基　　金：国家燕麦荞麦现代农业产业技术体系专项"荞麦育种岗位专项资金"(CARS-08-A4);国家自然科学基金项目"荞麦属植物落粒性相关基因研究"(31060207);"荞麦落粒性的遗传规律及其基因序列研究"(31171609);贵州百十千人才计划

摘　　要：为获得荞麦落粒性相关基因,分别选取甜荞、苦荞和金荞的不同品种不同组织混合样品进行转录组测序,通过数据库比对、功能分析与功能注释获取与落粒性相关的Unigene。设计引物通过RACE方法扩增候选基因,测序后将候选基因全长序列提交到NCBI数据库中,通过blast寻找候选基因的命名,用实时定量荧光PCR测候选基因的表达量。结果表明:得到6个候选基因,其中,根向光性蛋白基因、SOBIR1-like蛋白基因、NPR5-like蛋白基因主要在花器官的发育和形成过程中起调控作用,而SRG1-like蛋白基因、过氧化物酶基因和AGL蛋白基因主要在植物离区的形成和发育过程中起调控作用。NPR5-like蛋白基因在落粒品种与不落粒品种间的表达量差异最大。结论:NPR5-like蛋白基因在荞麦落粒过程中起一定的调控作用。Six candidate genes related to seed-holding character of buckwheat were determined from different tissue of F.esculentum,F.tataricum and F.dibotrys by RT-PCR to obtain genes related to seed-holding character of buckwheat.The results showed that root phototropism protein gene,SOBIR1-like protein gene,and NPR5-like gene have the regulating effect on the floral organ development and formation process,but SRG1-like protein gene,peroxidase gene and AGL protein gene have the regulating effect on the development and formation process of abscission zone in buckwheat mainly. There is significant difference in expression quantity of NPR5-like gene between shattering buckwheat variety and non-shattering buckwheat variety,which indicates that NPR5-like protein gene has a certain regulating effect on seed shattering process in buckwheat.

关 键 词：荞麦 落粒性 转录组 实时定量荧光PCR 

分 类 号：S517[农业科学—作物学]