妇科肿瘤相关间充质干细胞研究进展  

Research progress of gynecological oncology associated mesenchymal stem cells

作　　者：路春华[1] 盛修贵[1] 

机构地区：[1]山东省肿瘤医院妇瘤科,山东济南250117

出　　处：《中华肿瘤防治杂志》2015年第12期989-992,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金(2009ZRC03139);山东省医学科学院青年基金(2012-19);山东省肿瘤医院科研启动基金(2013-13)

摘　　要：目的总结目前妇科肿瘤相关间充质干细胞(mesenchymal stem cells,MSCs)的研究进展。方法应用PubMed和CNKI期刊全文数据库检索系统,以"宫颈肿瘤、卵巢肿瘤、子宫体肿瘤、外阴肿瘤、阴道肿瘤、输卵管肿瘤和间充质干细胞"为关键词,检索2000-01-01-2014-10-31的相关文献,纳入标准:1)MSCs相关文献;2)正常组织来源的MSCs与肿瘤;3)肿瘤组织中的MSCs;4)妇科肿瘤组织中的MSCs。根据纳入标准符合分析的文献29篇。结果 1)MSCs是一种机体内部间质内广泛存在的非造血系成体干细胞,可分化为骨骼、软骨和脂肪细胞等。2)正常组织来源的MSCs能调控肿瘤生长,起抑制还是促进作用与宿主免疫功能状态、肿瘤细胞类型和所处微环境等因素有关。肿瘤相关的炎症环境能改变正常MSCs的功能,使之获得高效招募炎性单核及巨噬细胞的能力,从而达到促进肿瘤生长的目的。MSCs能分化为成纤维细胞,为肿瘤进展提供结构和功能支持,形成血管细胞,促进微血管形成,形成间质网络改善肿瘤微环境,从而促进肿瘤的增殖。3)肿瘤组织中也存在MSCs,在肿瘤增殖调控中起重要作用,在肺癌、胃癌和骨肉瘤等实体瘤中得到分离和验证。4)妇科常见恶性肿瘤中的宫颈癌、卵巢癌和子宫内膜癌组织中均能分离出具有多向分化潜能的MSCs。宫颈癌组织标本的MSCs亚群,形态似成纤维细胞,呈克隆样增殖,免疫表型为CD13、CD29、CD44、CD105和HLA-I阳性,细胞保持正常的核型,在适当的诱导条件下,这些细胞能够分化成骨细胞等;宫颈癌相关MSCs能促进肿瘤细胞增殖,机制与血管生成和上皮间质转化相关。卵巢癌腹水和肿瘤组织中均可分离出MSCs,高表达CD44、MMP9和Oct4,具有正常的形态和核型,能分化成脂肪、软骨和骨,本身无致瘤性,能通过增加肿瘤干细胞的数目以促进肿瘤的生长。子宫内膜基质中的MSCs可以长期增殖,有分化和克隆形成OBJECTIVE To summarize the progress in study of gynecological oncology associated mesenchymal stem cells.METHODS Relative articles from Jan.1,2000 to Oct.31,2014 were searched in PubMed and CNKI journal with uterine cervix neoplasms,ovarian neoplasms,endometrial neoplasms,vulvar neoplasms,vagina tumor,tumor of the fallopian tube and mesenchymal stem cells as key words.Inclusion criteria：1）mesenchymal stem cells（MSCs）.2）MSCs and tumor.3）MSCs derived from tumor.4）Gynecological oncology associated MSCs.Finally 29 articles were collected.RESULTS 1）MSCs represent a non-hematopoietic and heterogeneous population in nearly all kinds of tissues,which show the capacity of self-renew and differentiation into adipose,cartilage and bone.2）Normal tissue derived MSCs shows both tuomor-inhibitory and tumor-promoting activities in variety of reports,which might be related in part to different host immune function,different tumor type and cellular microenvironments.MSCs could be recruited to the tumor microenvironment,however,controversy exists regarding their roles in solid tumors.3）MSCs isolated from lung cancer,stomach cancer and osteosarcoma played an important regulatory role in the of tumor growth.4）tumor tissues of MSCs were successfully isolated and identified from human uterine cervix cancer tissues.Those MSCs displayed fibroblast-like morphology and grew into colonies and kept a normal karyotype and typical MSCs phenotype,which could differentiate into osteogenic cells,adipogenic cells and hepatocytes under appropriate induction conditions.Co-culture of MSCs with cervical cells showed increased proliferation characteristics and enhanced tumor growth in vivo,which may be related to angiogenesis and EMT mechanisms.Carcinoma-associated MSCs（CA-MSCs）were identified in human ovarian ascites and tumor samples,which had normal morphologic appearance and normal karyotype.CA-MSCs were nontumorigenic and multipotent,they promote tumor growth by increasing the number of cancer stem cells through BMP signa

关 键 词：间充质干细胞 宫颈肿瘤 卵巢肿瘤 子宫内膜肿瘤 综述文献 

分 类 号：R737.3[医药卫生—肿瘤]