乳腺癌肿瘤微环境中HMGB1、miRNAs的检测及相关性分析  

Correlation between HMGB1 and mi RNAs in tumor microenvironment of breast cancer

作　　者：倪萍[1] 葛藤[1] 林新[2] 刘月琴[2] 包婷郡 谢婵娟[1] 张盼[1] 沈慧玲[2] 许文林[2] 许化溪[1] 苏兆亮[1] 

机构地区：[1]江苏大学免疫学研究所,镇江212013 [2]江苏大学第四附属医院中心实验室,镇江212001

出　　处：《免疫学杂志》2015年第6期501-504,共4页Immunological Journal

基　　金：江苏省六大人才高峰项目(2013-WSN-002);江苏省临床医学专项(BL2012059);江苏省妇幼课题(F201350);大学生课外学术科研项目(13A250);江苏大学研究生科研创新计划(KYXX0032)

摘　　要：目的检测mi R-21-5p、mi R-155-5p、mi R-218-5p、mi R-222-3p、mi R-494-3p和HMGB1在乳腺癌荷瘤小鼠肿瘤微环境中的表达水平,分析它们在乳腺癌发生、发展中的作用。方法收集乳腺癌MCF-7荷瘤小鼠手术标本;实时荧光定量PCR检测18只乳腺癌荷瘤小鼠手术标本中mi R-21-5p、mi R-155-5p、mi R-218-5p、mi R-222-3p、mi R-494-3p和HMGB1的m RNA的表达水平;Western blot法检测HMGB1在癌组织及癌旁组织中蛋白表达情况。结果与癌旁组织相比,肿瘤组织中mi R-21-5p、mi R-222-3p表达上调(P<0.05),mi R-155-5p、mi R-218-5p、mi R-494-3p表达下调(P<0.05);与癌旁组织相比,HMGB1在癌组织中高表达(P<0.05)。相关性分析发现,mi R-21-5p、mi R-222-3p与HMGB1的表达成正相关,而mi R-155-5p、mi R-218-5p、mi R-494-3p与HMGB1的表达成负相关。结论肿瘤微环境中mi R-21-5p、mi R-155-5p、mi R-218-5p、mi R-222-3p、mi R-494-3p和HMGB1可能在乳腺癌的发生、发展中起一定作用。This study designed to investigate the levels of mi R-21-5p, mi R-155-5p, mi R-218-5p, mi R-222-3p, mi R-494-3p and HMGB1 in tumor microenvironment of breast cancer and explore their clinical significance and correlations. Firstly, the surgical specimens of tumor-bearing mice were collect. RT-q PCR was employed to measure the m RNAs expression of mi R-21-5p, mi R-155-5p, mi R-218-5p, mi R-222-3p, mi R-494-3p and HMGB1 in tumor tissues and tumor-adjacent normal tissues from 18 tumor-bearing mice; Western blot was used to detect HMGB1 protein expression in tumor tissues and tumor-adjacent normal tissues from tumor-bearing mice.Data showed that comparing with the normal tissues, tumor tissues were characterized by over-expression of mi R-21-5p, mi R-222-3p（P〈0.05）, and by down-expression of mi R-155-5p, mi R-218-5p and mi R-494-3p（P〈0.05）.Additionally, tumor tissues were characterized by over-expression of HMGB1（P〈0.05）. The over-expression of mi R-21-5p, mi R-222-3p and the up-regulation of HMGB1 were in positive correlation, but mi R-155-5p, mi R-218-5p and mi R-494-3p negatively correlated with HMGB1 in tumor microenvironment of breast cancer. In conclusion, all the result suggested that mi R-21-5p, mi R-155-5p, mi R-218-5p, mi R-222-3p, mi R-494-3p might play important roles in the carcinogenic process of breast cancer.

关 键 词：微小RNA HMGB1 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]