重症急性胰腺炎大鼠回肠肌间神经节的VIP和NOS阳性神经元增加  

Ileum myenteric ganglia VIP and NOS positive neurons increased in rats with severe acute pancreatitis

作　　者：段泽辉[1] 林中[1] 张诚[1] 王芹[1] 

机构地区：[1]桂林医学院附属医院消化科,广西桂林541001

出　　处：《华夏医学》2015年第1期16-20,共5页Acta Medicinae Sinica

基　　金：广西卫生厅中医药科技专项课题资助(GZKZ10-125)

摘　　要：目的:观察重症急性胰腺炎(SPA)对大鼠胃肠动力和肠神经系统回肠肌间神经节血管活性肠肽(VIP)和一氧化氮合酶(NOS)阳性神经元的影响。方法:雄性大鼠(SD)20只随机分为假手术组和SAP组,用5%牛黄胆酸钠逆行胰胆管穿刺注射制作SAP模型。24h后,胃内灌服1%台盼蓝溶液,测定小肠推进比。应用双重免疫荧光染色分析肌间神经节VIP和NOS阳性神经元。结果:SAP组胰腺病理评分增高,大鼠小肠推进比率(30.20±3.82)%,低于假手术组(51.37±5.04)%,差异有统计学意义(P<0.05);SAP组肌间神经节VIP和NOS阳性神经元,高于假手术组,差异有统计学意义(P<0.05)。结论:重症急性胰腺大鼠胃肠动力障碍可能与抑制性神经元VIP和NOS的表达上调相关。Objective：To investigate the effects of severe acute pancreatitis （SPA） on gastrointestinal motility and ganglia ileum muscle vasoactive intestinal peptide （VIP） and nitric oxide synthase （NOS） positive neurons in rats. Methods： 20 Sprague Dawley（SD） rats were randomly divided into two groups： sham operated group and SAP group. SAP model was established by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. 24 h after the injection, stomach was filled with 1% trypan blue, then small intestine propulsion ratio was measured. Use double immunofluorescence to analyze myenteric ganglia VIP and NOS immunoreactive neurons. Results： In the SAP group, the histological score of the pancreas was increased, small intestine propulsion ratio （30.20±3.82） was lower than the sham operated group（51.37±5.04 ）% ,the difference was statistically significant（P〈0.05）;the myenteric ganglia VIP and NOS immunoreactive neurons percentage in the SAP group was higher than the sham operated group（P〈0.05）. Conclusion： Gastrointestinal motility disorder of severe acute pancreatitis rat may be correlated with the upregulated expression of VIP and NOS in inhibitory neurons.

关 键 词：肠神经系统 血管活性肠肽 重症急性胰腺炎 胃肠动力障碍 

分 类 号：R576[医药卫生—消化系统]