芒柄花黄素对人肺癌细胞A549增殖与凋亡的影响及其机制  

Effects and mechanism of formononetin on the proliferation and apoptosis of human lung cancer cells A549

作　　者：李涛 

机构地区：[1]曲靖市第一人民医院肿瘤科,云南曲靖655000

出　　处：《华夏医学》2015年第1期28-31,共4页Acta Medicinae Sinica

摘　　要：探讨芒柄花黄素对人肺癌细胞A549增殖和凋亡的影响及作用机制。方法:20,40,80μmol/L的芒柄花黄素作用于人肺癌细胞A549后,采用MTT法分析芒柄花黄素对A549细胞增殖的影响;流式细胞术检测芒柄花黄素对A549细胞凋亡的影响,采用RT-PCR检测芒柄花黄素对A549细胞Bcl-2mRNA的表达。结果:经芒柄花黄素作用后,MTT结果显示A549细胞的生长抑制率明显增加,流式细胞术检测显示A549细胞凋亡率升高,此外,RT-PCR检测A549细胞Bcl-2mRNA表达水平明显降低。结论:芒柄花黄素诱导肺癌细胞A549凋亡,抑制其生长,这可能与其下调Bcl-2mRNA表达有关。Objective：To observe the effect of formononetin on the proliferation and apoptosis of A549 cells and study the possible mechanism. Methods： Human lung cancer cells A549 were treated with different doses of formononetin 20, 40 and 80 μmol/L. MTT assay was applied to determine the effect of formononetin on proliferation of A549 cells, while apoptosis was assessed by flow cytometry. The expression levels of Bcl-2 mRNA in A549 cells were analyzed by RT-PCR. Results=After formononetin treatment, the proliferation of A549 cells was significantly inhibited, with increased apoptotic rates by flow cytometry. Furthermore, formononetin resulted in reduced expression of Bcl-2 mRNA in A549 cells. Conclusion： Formononetin inhibits the growth of human lung cancer cells A549 and induces it＇s apoptosis, which may be related with inhibition of Bcl-2 mRNA expression.

关 键 词：芒柄花黄素 肺癌 凋亡 BCL-2MRNA 

分 类 号：R734.2[医药卫生—肿瘤]