ECT2基因在胃癌组织中的表达及意义  

Significance of expression of ECT2 in gastric cancer

作　　者：严海翠[1] 王红兵[2] 

机构地区：[1]徐州医学院研究生学院,江苏省徐州市221002 [2]徐州医学院附属医院肿瘤科,江苏省徐州市221002

出　　处：《世界华人消化杂志》2015年第14期2215-2220,共6页World Chinese Journal of Digestology

摘　　要：目的:检测胃癌组织及相应的癌旁组织中上皮细胞转化序列2癌基因(epithelial cell transforming sequence 2 oncogene,ECT2)的蛋白及m RNA表达情况,并探讨其与胃癌临床病理因素的相关性及临床意义.方法:采用免疫组织化学S-P法及逆转录聚合酶链反应(RT-PCR)法检测50例胃癌患者的癌组织及相应的癌旁组织(距癌组织边缘>5 cm)中ECT2蛋白及m RNA表达情况.结果:免疫组织化学结果显示,胃癌组织中ECT2蛋白阳性表达率(76.0%)显著高于癌旁组织(36.0%)(P<0.01);RT-PCR结果显示,癌组织中ECT2 mR NA表达水平显著高于癌旁组织(P<0.05).ECT2蛋白及m RNA表达均与胃癌的分化程度、TNM分期及淋巴结转移密切相关(P<0.05),与患者的性别、年龄、肿瘤直径无明显相关(P>0.05);胃癌组织中ECT2蛋白表达与mR NA表达之间呈显著正相关(P<0.01).结论:ECT2蛋白及m RNA在胃癌组织中均呈高表达,且与胃癌分化程度、TNM分期及淋巴结转移密切相关;ECT2在胃癌的发生发展中起重要作用,可以作为反映胃癌生物学行为的有效指标.AIM： To investigate the expression of epithelial cell transforming sequence 2（ECT2） in gastric cancer（GC） and to analyze its correlation with clinicopathological characteristics. METHODS： Immunohistochemistry（IHC） and RT-PCR were used to examine the expression of ECT2 protein and m RNA in GC tissues and paired adjacent normal tissues. The correlation between ECT2 expression and clinicopathological characteristics was then analyzed.RESULTS： In comparison with adjacent normal tissues（36.0%）, the expression of ECT2 protein in GC tissues（76.0%） was significantly higher（P〈0.01）, and the expression of ECT2 mR NA in GC tissues was also significantly higher in GC tissues than in adjacent normal tissues. The expression of ECT2 protein and m RNA was related to histologic differentiation, TNM stage and lymph node metastasis in GC（P〉0.05）, but not to gender, age or tumor size（P〈0.05）. CONCLUSION： Both ECT2 protein and mR NA are overexpressed in GC, and ECT2 expression correlates with the histologic differentiation, TNM stage and lymph node metastasis in GC. ECT2 may play an important role during GC progression, and may serve as a good factor to indicate biologic behavior of GC.

关 键 词：胃癌 上皮细胞转化序列2 免疫组织化学 逆转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]