气相色谱-质谱法同时分析运动营养品中的大麻酚、大麻二酚和Δ9-四氢大麻酚  

Simultaneous Determination of the Cannabinol, Cannabidiol and Δ9-Tetrahydrocannabinol in Athletic Nutrition by Gas Chromatography-mass Spectrometry

作　　者：王占良[1] 张建丽[1] 张亦农[1] 

机构地区：[1]国家体育总局反兴奋剂中心食品药品兴奋剂检测实验室,北京100029

出　　处：《中国运动医学杂志》2015年第4期398-403,共6页Chinese Journal of Sports Medicine

摘　　要：目的：建立气相色谱-质谱法同时分析运动营养品中大麻酚、大麻二酚和Δ9-四氢大麻酚的检测方法。方法：样品采用液液萃取,提取液经过正相固相萃取柱（Silica）纯化,洗脱液氮气吹干,N-甲基-N-（三甲基硅烷）三氟乙酰胺衍生化后,采用HP-1MS柱（17 m×0.2 mm i.d.×0.11 mm）色谱分离,程序升温,质谱检测,D3-Δ9-四氢大麻酚为内标。结果：大麻酚、大麻二酚和Δ9-四氢大麻酚的衍生化产物实现基线分离,三个定性特征离子的峰度比计算结果可以满足世界反兴奋剂机构（WADA）要求。该方法的检测限为1μg/kg,定量限为2μg/kg,在添加的高、中、低三种浓度的相对回收率分布在94%~119%之间。结论：该方法满足常规检测要求,样品前处理简单、快速、可靠。Objective To introduce a method for determining cannabinol, cannabidiol and Δ9-tetrahydrocannabinol by gas chromatography-mass spectrometry（GC/MS） simultaneously. Methods The target compounds were prepared by liquid-liquid phase extraction and purified by Silica Oasis solid prepared extraction column, the elution was dried by nitrogen, the analytes were completely separated by HP-1MS column（17m ×0.2mm i.d. ×0.11μm thickness） in linear temperature program and detected by GC-MS.Quantitative analysis was corrected by an isotope internal standard D3-Δ9-tetrahydrocannabinol. Results The relative recovery of target compounds spiked in blank sample in high,moderate and low concentrations ranged from 94 to 119%. 1μg/kg of detection limits for target analytes and 2μg/kg of quantification limits were achieved. Conclusion The method introduced in this study is simple, rapid, reliable and suitable for routine analysis of athletic nutrition. 【Key Words】：

关 键 词：大麻酚 大麻二酚 Δ9-四氢大麻酚 运动营养品 气相色谱-质谱法 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.63[医药卫生—公共卫生与预防医学]