肿瘤坏死因子相关诱导凋亡配体及其受体3、4在结直肠癌组织中的表达及意义  

The expression of tumor necrosis factor - related apoptosis - inducing ligand （TRAIL） protein and it＇ s receptor 3,4 in coiorectal cancer tissue and its significance

作　　者：范广民[1] 阮正英[2] 陈豪[2] 

机构地区：[1]台州市中心医院病理科,浙江省台州317000 [2]台州市中西医结合医院病理科

出　　处：《中国基层医药》2015年第11期1639-1643,J0002,共6页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省温岭市科技计划项目（2014-1-65）

摘　　要：目的探讨肿瘤坏死因子相关诱导凋亡配体（TRAIL）信号通路在结直肠癌发病机制中的作用。方法取41例结直肠癌组织（结直肠癌组）和癌旁正常组织（正常组），免疫组化法测定两组组织中TRAIL及TRAILR3、TRAILR4蛋白的表达。结果结直肠癌组织TRAIL、TRAILR3的阳性率与正常组差异均无统计学意义（88％与90％；95％与93％，）f2=0．125、0．213，均P〉0．05），但两者的阳性表达强度显著低于正常组[（0．237±0．036）与（0．289±0．069）；（0．226±0．052）与（0．281±0．068），t=4．125、4．025，均P〈0．01]；两者的阳性表达强度在低分化癌组织中显著低于高中分化癌[（0．205±0．021）与（0．245±0．034）；（0．185±0．032）与（0．236±0．051），t=4．025、2．664，P〈0．01、P〈0．05]，但与淋巴结转移无关[（0．235±O．035）与（0．241±0．338）；（0．216±0．048）与（0．231±0．054），t=0．448、0．862，均P〉0．05]。结直肠癌组TRAILR4蛋白的阳性率和阳性表达强度与正常组差异均无统计学意义[93％与98％；（0．196±0．085）与（0．219±0．061），χ^2=1．051、t=1．353，均P〉0．05]，还与癌分化程度、淋巴结转移无关[（0．176±0．052）与（0．201±0．091）；（0．194±0．054）与（0．197±0．100），t=0．667、0．448，均P〉0．05]。结论TRAIL和TRAILR3在结直肠癌组织中表达下降，且与Gleason分级相关，它们可作为结直肠癌的预后指标。Objective To investigate the potential roles of tumor necrosis factor - related apoptosis - indu- cing ligand（TRAIL） signaling system in the pathogenesis of colorectal cancer. Methods Immunohistochemistry tech- niques were used,the TRAIL and its receptor（TRAILR3, TRAILR4） protein were analyzed in both 41 colorectal cancer samples （ colorectal cancer group） and normal samples beside cancer tissue （normal group）. Results The lev- els of TRAIL and TRAILR3 protein expression in the colorectal cancer group were significantly lower than those in the normal group[ （0.237 ±0.036）vs. （0.289±0.069） ;（0.226±0. 052） vs. （0. 281 ±0. 068） ,t =4. 125,4. 025,all P 〈 0.01 ] ,while the positive rate of them were nonsignificant difference between the two groups（88% vs. 90% ;95% vs. 93% ,χ^2 =0. 125,0. 213, all P 〉 0.05）. The expression levels of them in the coloreetal cancer group with poorl differentiation were notably lower than those with high - moderate differentiation [ （ 0. 205 ± 0. 021 ） vs （ 0. 245 ± 0. 034 ） ; （0. 185 ± 0.032） vs （0. 236 ±0.051 ）, t = 4. 025,2. 664, P 〈 0.01, P 〈 0.05 ], but they were not strongly correlated to lymph nodes metastasis [ （0. 235 ± 0. 035 ） vs （0. 241± 0. 338） ; （ 0. 216 ± 0. 048 ） vs （0. 231 ±0. 054）, t = 0. 448,0.862, all P 〉 0.05 ]. Moreover, the positive rate and expression levels of TRAILR4 protein was non- statistic significant difference between the two groups[93% vs 98% ;（0. 196 ±0.085 ） vs （0. 219 ± 0. 061 ） , χ^2 = 1.051, t = 1. 353, all P 〉 0.05 ] , and it was also non - significantly correlated with cancer cell differentiation and lymph nodes metastasis[ （0. 176±0.052） vs （0. 201 ±0. 091 ） ;（0. 194 ±0. 054） vs （0. 197 ±0. 100） ,t =0. 667, 0. 448, all P 〉 0.05 ]. Conclusion The levels of TRAIL and TRAILR3 expression are attenuated at colorectal cancer tissue. The expression of them are correlated with cancer cell differentiation grade. These findings indicat

关 键 词：结直肠肿瘤 TNF相关凋亡诱导配体 信号传导 

分 类 号：R735.34[医药卫生—肿瘤]