内循环半短程亚硝化工艺运行条件与微生物群落研究  

Running Condition and Bacterial Community Associated with the Partial Nitritation System

作　　者：赵志瑞[1,2,3] 焦海华[4] 崔丙健[3] 黄迪[1] 曹世超[1] 王云[1] 刘上千 马斌[5] 白志辉[3] 

机构地区：[1]石家庄经济学院水资源与环境学院,石家庄050031 [2]河北省水资源可持续利用与开发重点实验室,石家庄050031 [3]中国科学院生态环境研究中心,北京100085 [4]长治学院生物科学与技术系,长治046011 [5]北京工业大学市政环境工程学院,北京100124

出　　处：《环境科学》2015年第4期1399-1405,共7页Environmental Science

基　　金：中国科学院重点部署项目(KZZD-EW-09-3);国家水体污染控制与治理科技重大专项(2012ZX07203-006);水利部公益性行业科研专项(201401078)

摘　　要：在内循环半短程亚硝化工艺中，污泥浓度为4000mg·L^-1、溶解氧小于0．2mg·L^-1、温度（15～29℃）、水力停留时间4．6h条件下，不同的回流比对系统中微生物群落有着明显的影响，回流比75％时，微生物的生物量达到最高值，出水中的亚硝态氮和氨氮的浓度比可控制在1．定量PCR和16SrRNA基因的克隆文库结果表明：在低溶解氧浓度下氨氧化菌是主要脱氮菌群，该菌群促进了半短程亚硝化反应的进行，与传统的硝化系统比较，在内循环半短程亚硝化工艺中没有检测到硝化螺旋菌（Nitrospira）和硝化杆菌（Nitrobacter），在内循环半短程亚硝化系统中浮霉菌属（Planctomycetes）的量也高于传统的脱氮系统．氨单加氧酶基因克隆文库结果表明，系统中的氨氧化菌群主要属于亚硝化单胞菌属（Nitrosomonas）．因此内循环半短程亚硝化工艺在经济和技术上是可行的．In partial nitritation process, the sludge concentration was higher than 4000 mg·L^-1 , DO was less than 0. 2 mg·L^-1 , the temperature of 15-29℃ , the reactor HRT of 4.6 h, and the circulation ratio was 75% , not only the microbial biomass reached the highest value but also the ratio of nitrite to ammonium of the effluent could be controlled at approximate 1.0 by adjusting the dissolved oxygen concentration. A quantitative PCR and the 16S rRNA genes clone library results demonstrated that ammonia-oxidizing bacteria were the dominant among nltrite-oxidizing bacteria at low dissolve oxygen concentration, that the bacteria promoted the running of the partial nitritation. Compared with conventional nitrification systems, in partial nitritation process, Nitrobacter and Nitrospira could not be detected among the system, and abundance of Planctomycetes was higher in partial nitritation process than that of conventional nitrification systems. Phylogenetic analysis of bacterial amoA gene sequences suggested that ammonia-oxidizing bacteria of the partial nitritation system was dominated by Nitrosomonas sequences, so partial nitritation is to be feasible technically and economically.

关 键 词：半短程亚硝化 氨氧化菌 定量PCR 克隆文库 氨单加氧酶基因 

分 类 号：X172[环境科学与工程—环境科学] X703.1