HPLC-ESI-MS和FTIR方法鉴别不同产地中药肉苁蓉的研究  

Identification of Chinese Traditional Medicine Cistanches Herba from Different Places by HPLC-ESI-MS and FTIR Methods

作　　者：周晔[1,2] 李薇[1] 韩立峰[1] 宋新波[1,3] 李佩孚[2] 汪蕊[2] 张伯礼[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津医科大学药学院,天津市临床药物关键技术重点实验室,天津300070 [3]内蒙古曼德拉沙产业开发有限公司,内蒙古阿拉善左旗750306

出　　处：《光谱学与光谱分析》2015年第4期1056-1061,共6页Spectroscopy and Spectral Analysis

基　　金：天津市科技创新专项资金项目(08FDZDSH01400);国家科技支撑计划项目(2007BAI47B00)资助

摘　　要：采用HPLC-ESI-MS和傅里叶红外光谱法对五种不同产地中药肉苁蓉进行了分析,采用HPLCESI-MS检测了中药肉苁蓉中荒漠肉苁蓉苷A、松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、2’-乙酰基毛蕊花糖苷、荒漠肉苁蓉苷C、管花苷B七种有效成分的含量。采用傅里叶红外光谱法,以五种药用植物样品的红外指纹图谱为依据,计算出所测样品的共有峰率和变异峰率。建立了五种药用植物的共有峰率和变异峰率双指标序列。结果显示两种方法对药材评价结果基本一致。采用傅里叶变换红外光谱法亦可区分肉苁蓉的常见伪品锁阳。HPLC-ESI-MS具有高通量、高灵敏度、分析快速等优点,可以通过主要化学成分含量的测定对肉苁蓉进行质量评价。傅里叶红外光谱法可以对样品进行无损处理,不需要对药材进行复杂的提取分离过程即可获得肉苁蓉红外光谱特征,其光谱的吸收峰强度与峰形是各种官能团互相作用的结果,是肉苁蓉多组分红外光谱的叠加。我们能从整体水平分析谱图特征峰,具有分析速度快,重现性好,非破坏性和样品量小,制样简单,专属性强,节约成本,保护环境等优点,符合中药向综合分析和整体分析的发展趋势。HPLC-ESI-MS和傅里叶红外光谱法联合应用,两种方法互为补充,为鉴别中药的真伪,探讨中药质量评价提供了一种新方法。Five samples of Cistanches Herba from different places were analyzed by HPLC‐ESI‐MS and FTIR methods .The effective compositions in Cistanches Herba including cistanoside A ,echinacoside ,acteoside ,isoacteoside ,2’‐actylacteoside , cistanoside C and tubluoside B were determined by HPLC‐MS .The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of the five samples and the dual index sequence of common peak ratio and variant peak ratio were established . The results showed that the evaluation results of the samples by the two methods were the same . The general fake plant Cynomorii Herba could be identified by FTIR .HPLC‐ESI‐MS ,which has high sensitivity and rapid determination procedure , can be used to evaluate quality of Cistanches Herba by quantitative analysis of the primary compositions .FTIR is a non‐destruc‐tive analysis method without complicated extraction and separation procedures to the samples .The absorption strength and the absorption shape were the synergistic effect of the functional groups and the nestification of the components in Cistanches Herba . The provided method has some advantages such as rapid analysis process ,good reproducibility ,non‐destructive ,small quantity of sample ,simple treatment ,good specificity ,low‐cost and environment‐friendly .The method meets the trend of complex analy‐sis and whole analysis for the Chinese medicines .Combination of FTIR and HPLC‐ESI‐MS was a good method for identification and evaluation of quality of Chinese medicines .

关 键 词：肉苁蓉 HPLC-ESI-MS 红外指纹图谱 双指标法 质量评价 

分 类 号：O657.3[理学—分析化学]