沙拉沙星ELISA快速检测试剂盒的研制及性能鉴定  

Development and application of rapid test ELISA Kit in Sarafloxacin

作　　者：刘儒彪[1,2] 胡骁飞[2] 张改平[3] 宋照军[1] 王方雨[2] 邓瑞广[2] 刘璐[1] 刘玺[1] 

机构地区：[1]河南科技学院食品学院,河南新乡453003 [2]河南省农业科学院河南省动物免疫学重点实验室,河南郑州450002 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《食品与发酵工业》2015年第2期189-194,共6页Food and Fermentation Industries

基　　金：河南省高校科技创新团队支持计划资助(13IRTSTHN006);国家科技支撑计划畜禽产品安全生产监控和检测技术研究与示范(2014BAD13B05);2015年河南省科技攻关计划项目(动物源食品SARA残留的ELISA快检技术)

摘　　要：利用已制备的高亲和力的抗沙拉沙星的单克隆抗体,研制沙拉沙星快速检测试剂盒,并对其性能进行鉴定。结果显示:研制的沙拉沙星试剂盒的标准曲线呈S型,符合4参数logit曲线拟合,相关线性方程为y=-0.384 8x+0.736 3,R2=0.990 1,IC50为4.11 ng/m L,LOD为2.06 ng/m L。与其他药物的交叉反应率(CR%)均低于1%;鸡肝和鸡肉中添加平均回收率分别为85.3%和88.7%,且变异系数均小于15%;试剂盒在4℃条件下保存6个月后检测能力无显著变化。Via using prepared monoclonal antibodies that has high affinity to develop a ELISA test kit for detecting Sarafloxacin （SARA） and identify its characteristic. The calibration curve of SARA-Kit with standard SARA inhibitor was a typical sigmoid curve fitted to four parameter logistic equation with the linear detection of y = -0. 384 8x ＋ 0. 736 3 （R^2 = 0. 990 1 ） , the IC50 was 4. 11 ng/mL and LOD was 2.06ng/mL. The SARA-Kit cross-reactivity towards other compounds were less than 1%. The recoveries of SARA spiked in chicken livers were 85.3% ,in chicken meat were 88.7%. The coefficient variation were all less than 15%. The validity of SARA-Kit in 4℃ for six months had little change.

关 键 词：沙拉沙星 单克隆抗体 ELISA试剂盒 快速检测 

分 类 号：S859.79[农业科学—临床兽医学]