基于iTraq技术的加拿大一枝黄花提取物作用下铜绿微囊藻细胞差异表达蛋白  

Differentially expressed proteins in Microcystic aeruginosa with Solidago canadensis L. extracts using iTraq labeling technique

作　　者：黄莹莹[1] 白羽[1] 王艳[1,2] 孔海南[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]上海交通大学公共卫生学院,上海200025

出　　处：《中国环境科学》2015年第6期1822-1830,共9页China Environmental Science

基　　金：国家自然科学基金(21077074)

摘　　要：为研究加拿大一枝黄花提取物对藻类生长抑制作用的分子机理,应用iTraq技术结合LC-MS-MS,分析了铜绿微囊藻细胞蛋白质的差异表达.共鉴定出261种差异蛋白(变化倍数≥1.5),其中表达上调的220种,表达下调的41种.GO功能分类显示,加拿大一枝黄花提取物通过影响细胞代谢过程、蛋白质催化和结合功能等方式抑制藻细胞生长.KEGG通路分析表明,通路大类中富集程度位于前3位的均是代谢通路,包括能量代谢、碳水化合物代谢和氨基酸代谢,通路中富集程度最高的是糖酵解/糖异生通路.本研究发现了加拿大一枝黄花提取物对藻细胞生长抑制作用的分子靶点,为从分子层面阐明其抑藻作用提供参考,也为研究植物化感物质的抑藻机理提供了新方法.To investigate antialgal mechanism Of Solidago canadensis L. extracts, iTraq labeling technique coupled with LC-MS-MS was used to analyze differentially expressed proteins in Microcystic aeruginosa. 261 differentially expressed proteins （fold change ratio〉1.5） were identified, in which 220 proteins were upregulated and 41proteins were down-regulated. GO function analysis showed that the extracts of S. canadensis inhibited the algal growth by affecting metabolism process, protein catalytic activity and binding function. KEGG pathway analysis showed that top 3 enrichment pathway classes were all related to metabolism, including energy, carbohydrate and amino acid metabolism, and glycolysis/gluconeogenesis was the top enrichment pathway. This study elucidated the molecular targets of the extracts orS. canadensis, thereby giving insight into the antialgal effects at the molecular level and providing a new way to study the antialgal mechanism of plant allelopathic compounds.

关 键 词：iTraq标记 化感作用 差异表达蛋白组 抑藻机理 

分 类 号：X172[环境科学与工程—环境科学]