皱皮木瓜皮渣齐墩果酸、熊果酸和总黄酮连续提取工艺研究  

Co-Production Extraction Process for Oleanolic Acid,Ursolic Acid and Total Flavonoids from the Peel Pomace of Chaenomeles speciosa(Sweet)Nakai

作　　者：卞京军 程密密 刘世尧[1] 白志川[1] 韦正鑫 蔡娟[1] 

机构地区：[1]西南大学园艺园林学院/南方山地园艺学教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2015年第3期158-165,共8页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科技攻关计划项目(CSTC;2009AC1180)

摘　　要：采取高效液相色谱法与三氯化铝比色法检测皱皮木瓜皮渣齐墩果酸、熊果酸和总黄酮得率,通过单因素试验进行3种物质提取因素敏感区域确定,并利用正交试验设计进行3种物质连续提取工艺指标优化研究.结果表明:1齐墩果酸、熊果酸:超声提取,乙醇体积分数95%,料液比为1∶10(g/mL),提取温度70℃,提取时间40min,此时得率最高,为1.677%;2总黄酮:超声提取,乙醇体积分数50%,料液比为1∶25(g/mL),提取温度60℃,提取时间70min,此时得率最高,为0.527%.Objective： To optimize the co-production extraction process for oleanolic acid （OA）, ursolic acid （UA） and total flavonoids from the peel pomace of Chaenomeles speciosa （Sweet） Nakai. Methods： A sin- gle factor test was conducted to determine the sensitive regions for the extraction of the above three sub- stances, and then an orthogonal test was used to optimize the factors of co-production extraction process： extracting time, temperature, the ratio of solid to liquid and solvent concentration. The contents of olean- olic acid and ursolic acid were determined by HPLC. Aluminium trichloride colorimetry was used to detect the total flavonoids. Results： The optimum conditions for ultrasonic extraction of OA and UA were as fol- lows： a yield of 1. 677% was obtained when the solvent concentration was 95% ethanol, the extracting time was 40 min, the extracting temperature was 70 ℃ and the ratio of materials to solution was 1 ： 10 （g/ mL） ; the optimum conditions for ultrasonic extraction of total flavonoids were as follows： the solvent con- centration was 50% ethanol, the extracting time was 70 min, the extracting temperature was 60 ℃ and the ratio of materials to solution was 1 ： 25 （g/mL）, and under such conditions the yield of total flavonoids was 0. 527%. Conclusion： This method was simple, convenient and accurate for the detection of oleanolic acid, ursolic acid and total flavonoids.

关 键 词：皱皮木瓜 齐墩果酸 熊果酸 高效液相色谱法(HPLC) 总黄酮 正交试验 

分 类 号：R284.2[医药卫生—中药学]