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作 者:王军[1] 蔡彩虹[1] 陈亮亮[1] 李益[2] 左文健[1] 梅文莉[1] 戴好富[1]
机构地区:[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]新疆农业科学院海南三亚农作物育种试验中心,乌鲁木齐830091
出 处:《中国实验方剂学杂志》2015年第12期26-30,共5页Chinese Journal of Experimental Traditional Medical Formulae
基 金:国家科技支撑计划项目(2013BAI11B04);公益性行业(农业)科研专项(201303117);海南省重大科技项目(ZDZX2013008-4)
摘 要:目的:分析海南山油柑茎皮和木质部的挥发油成分,并测试其生物活性。方法:采用水蒸气蒸馏法提取挥发油,进行气相色谱-质谱联用法(GC-MS)分析,采用NIST05和WILEY275L数据匹配进行鉴定。采用滤纸片琼脂扩散法测定抗菌活性,采用MTT法测定细胞毒活性。结论:海南山油柑茎木质部挥发油共鉴定出77种成分,占挥发油总量的67.74%,其主要成分为棕榈酸(18.84%),α-古巴烯(7.94%),δ-杜松烯(3.71%),(E,Z)-2,4-癸二烯醛(3.45%)和香树烯(3.30%)。茎皮挥发油共鉴定出75种成分,占挥发油总量的96.25%,其主要成分为α-蒎烯(46.70%),α-古巴烯(19.81%),δ-杜松烯(5.80%),香树烯(4.46%)和柠檬烯(3.53%)。木质部和茎皮挥发油中有39种相同的成分。茎皮挥发油对金黄色葡萄球菌的有抑制活性,而木质部挥发油无活性。细胞毒活性测试结果表明,山油柑茎皮和木质部挥发油对人慢性髓原白血病细胞K562,人胃癌细胞SGC-7901和人肝癌细胞SEL-7402的增殖均显示了较强的抑制活性,而茎皮挥发油的活性更好。Objective: To analyze the chemical constituents of volatile oil from the xylem and bark of Acronychia pedunculata in Hainan and its antibacterial activity and cytotoxicity. Method: The volatile oil was extracted by water-steam distillation and analyzed by GC-MS. The relative contents of the compounds were determined by normalization. The compounds were characterized by NIST05 and WILEY275L database matching. Antibacterial activities of the xylem and bark oil were assayed by the filter paper disc agar diffusion and the cytotoxicity of them was evaluated by MTT. Conclusion: 77 compounds were identified from the xylem oil which constituting 67.74% of the total oil and its main constituents were palmitic acid (18.84%), a-copaene (7.94%), 8-cadinene (3.71%), (E, Z) -:2, 4-decadienal (3.45%) and alloaromadendrene (3.30%). 75 compounds were identified from the bark oil which constituting 96.25% of the total oil and its main constituents were a-pinene (46.70%), a-copaene ( 19.81% ), 8-cadinene (5.80%), alloaromadendrene (4. 46% ), and limonene (3.53%). 39 compounds were identified both from the xylem oil and bark oil. The volatile oil from the bark showed antibacterial activity against Staphylococcus aureus, but the oil from xylem was inactive. The oil from xylem and bark showed significant cytotoxicactivities towards chronic myelogenous leukemia (K562), human hepatoma (SEL-7402) and human gastric carcinoma (SGC-7901). The activity of the former was better than the latter.
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