胰高血糖素样肽-1对高糖诱导的兔胸主动脉内皮细胞凋亡与增殖的影响  

Effect of glucagon-like peptide-1 on apoptosis and proliferation of rabbit thoracic aortic endothelial cells in high glucose

作　　者：蔡方刚[1] 郭平凡[1] 詹腾辉[1] 吴捷[1] 

机构地区：[1]福建医科大学附属第一医院血管外科,福建福州350004

出　　处：《中国现代医学杂志》2015年第16期7-10,共4页China Journal of Modern Medicine

基　　金：福建省教育厅科技项目(No:JA11117)

摘　　要：目的探讨胰高血糖素样肽-1(GLP-1)对高糖下兔胸主动脉内皮细胞凋亡与增殖变化的影响。方法取兔胸主动脉,用酶消化法分离内皮细胞,传代后于不同实验条件下培养,用TUNEL法测定各组胸主动脉内皮细胞凋亡,用MTT法与BRDU法测定各组胸主动脉内皮细胞的活力与增殖。结果与对照组相比,高糖作用下兔胸主动脉凋亡明显增多,而内皮细胞的活力下降、增殖减少(P<0.01),而用GLP-1预处理后,高糖抑制胸主动脉内皮细胞增殖与活力以及促进凋亡的作用明显减弱(P<0.05,与高糖组相比)。GLP-1的作用可以为GLP-1的受体抑制剂(exendin 9-39)所抵消,与高糖组相比,exendin9-39组的胸主动脉内皮细胞凋亡与细胞活力、增殖无明显变化(P>0.05)。结论胰高血糖素样肽GLP-1对高糖损害血管内皮细胞具有保护作用,其机制可能与其通过GLP-1受体途径促其增殖与抑制凋亡有关。[Objective] To observe the effect of glucagon-like peptide-1 （GLP-1） on the apoptosis and prolifera- tion of the rabbit thoracic aortic endothelial cells in high glucose. [ Methods ] Rabbit thoracic aortic endothelial cells were isolated from rabbit thoracic artery and cultured under different experimental conditions. Terminal deoxynu- eleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to assess endothelial cells apoptosis. Cell activity and proliferation was analyzed with MTT and BRDU. [ Results ] Compared with the control group, the apoptosis ratio of rabbit thoracic aortic endothelial cells cultured under high glucose （33mM） for 24 hours increased, but cell activity decreased and proliferation was inhibited （P 〈 0.01）. Pretreatment with GLP-1 in the high glucose group could attenuate the high glucose-induced effect （P 〈 0.05, compared with high glucose group）. The effect of GLP-1 on the rabbit thoracic aortic endothelial ceils was inhibited by exendin （9-39） （GLP-I receptor antagonist）. Compared with the high glucose group, there was no significant difference in the exendin（9-39） group in the apopto- sis and proliferation of rabbit thoracic aortic endothelial cells （P 〉0.05）. [ Conclusions ] GLP-1 can protect the rab- bit thoracic aortic endothelial cells from the high glucose-induced injury, which is related to the inhibition of en- dothelial cells apoptosis and the promotion of cell proliferation through GLP-1R-dependent pathways.

关 键 词：胰高血糖素样肽-1 高糖 胸主动脉内皮细胞 增殖 凋亡 

分 类 号：R-332[医药卫生]