Med-19基因在肾细胞癌中的表达及其与临床病理特征的关系  

Expression of Med-19 in renal cell carcinoma and its association with clinicopathologic features

作　　者：廖义翔[1] 周家杰[1] 魏嵬[2] 曾金敏[1] 杨光华[1] 陈忠军[1] 严友纪[1] 沈昊[1] 

机构地区：[1]华中科技大学同济医学院附属荆州医院泌尿外科,荆州434020 [2]华中科技大学同济医学院附属荆州医院放射介入科,荆州434020

出　　处：《中华实验外科杂志》2015年第6期1244-1246,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省荆州市科技局计划项目（2014039）

摘　　要：目的 检测Med-19基因在肾细胞癌组织中的表达水平,探讨肾细胞癌的生物学特征与其表达之间的关系.方法 应用免疫组织化学、反转录-聚合酶链反应（RT-PCR）及Western blot技术检测60例肾细胞癌组织、20例正常肾脏组织中Med-19基因的表达,并分析其与临床特征之间的关系.结果 免疫组织化学分析显示Med-19在正常肾脏组织中表达率为30.0％ （6/20）,而肾癌组织中Med-19的表达率为76.6％ （46/60）,提示Med-19基因在肾癌组织中显著上调（P＜0.05）;Western blot检测到Med-19蛋白表达的共48例,表达水平为0.618±0.125;但Med-19蛋白表达仅在2例正常肾脏标本中被检测到,其表达水平为0.076±0.023,差异有统计学意义（t=18.13,P＜0.05）;Med-19 mRNA的表达显示肿瘤标本中,50例检测到Med-19 mRNA的表达,其表达水平为0.863 ±0.081;Med-19 mRNA的表达仅在1例正常肾脏组织中被检测到,其表达水平为0.102±0.039,两者差异有统计学意义（t=18.98,P＜0.05）.肾细胞癌组织中Med-19基因mRNA和蛋白的表达水平显著高于正常肾脏组织（P＜0.05）;其表达水平与肿瘤的直径、临床分期、分化程度、有无淋巴结转移等生物学行为显著相关（P＜0.05）;与性别、年龄无明显相关（P＞0.05）;Spearman等级相关分析显示Med-19基因mRNA与蛋白表达呈正相关（r=0.776,P＜0.05）.结论 Med-19基因在肾细胞癌中过度表达,有助于判断肾细胞癌恶性转变以及肾细胞癌发生浸润转移.Objective To detect the expression of Med-19 gene mRNA and protein in renal cell carcinoma and to investigate the correlation between Med-19 gene expression and clinicalopathological features.Methods Med-19 gene was detected in 60 cases of renal cell carcinoma and 20 cases of normal renal tissues by reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blotting.The correlation between the Med-19 expression and clinicopathological features was analyzed.Results Immunohistochemical analysis showed that the expression rate of Med-19 in renal cell carcinomas was increased significantly [76.6％ （46/60）,P ＜ 0.05] as compared with that in normal renal tissues [30.0％ （6/20）].Western blotting showed that the Med-19 protein was detected in a total of 48 renal cell carcinomas with the expression level being 0.618 ± 0.125,and that was detected in only 2 cases of normal renal specimens with the expression level being 0.076 ± 0.023 （t =18.13,P ＜ 0.05）.Med-19 mRNA expression was detected in 50 renal cell carcinomas with the expression level being 0.863 ± 0.081,and that was detected in only 1 normal renal tissue with the expression level being 0.102 ± 0.039 （t =18.98,P ＜ 0.05）.Med-19 gene mRNA and protein expression levels in renal cell carcinoma tissues were significantly higher than those in normal renal tissues （P ＜ 0.05）.There were significant correlations between the expression level and the biological behaviours such as the diameter of the tumor,clinical stage,differentiation,biological behaviors,and lymph node metastasis （P ＜ 0.05）,but no correlations between the expression level with gender and age （P ＞ 0.05）.Spearman rank correlation analysis indicated that the expression of Med-19 gene mRNA was positively correlated with its protein （P ＜ 0.05）.Conclusion Overexpression of Med-19 gene in renal cell carcinoma may be associated with tumor progression.

关 键 词：肾癌 Med-19基因 

分 类 号：R737.11[医药卫生—肿瘤]