裸鼠肝癌模型中活化态肝星状细胞分泌血管生成素-1对肿瘤生长及微血管生成的影响  

Effects of activated hepatic stellate cells on proliferation and angiogenesis in nude mice hepatocellular carcinoma model through secretion of angiopoietin-1

作　　者：林楠[1] 唐亚军[1] 王旭天 方和平[1] 周正[1] 许瑞云[1] 

机构地区：[1]中山大学附属第三医院肝胆外科,广州510630

出　　处：《中华实验外科杂志》2015年第6期1317-1319,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81272642）;教育部博士点基金资助项目（20110171110070）

摘　　要：目的 利用裸鼠肝癌模型观察活化态肝星状细胞（aHSC）分泌血管生成素-1（Ang-1）对肝癌生长及微血管生成的影响.方法 建立裸鼠肝癌模型,实验组为肝星状细胞株（LX-2）与肝癌细胞株（HepG2）细胞混合注射（1∶1,100μl,细胞浓度：5 × 109/L）,干预组为Ang-1表达抑制的LX-2细胞株（LX-2-siAng-1）与HepG2细胞混合注射;对照组为HepG2细胞单独注射.4周后测量肿瘤体积,同时检测Ang-1、Ang-2与血管标志物CD34的mRNA与蛋白表达.结果 实验组肿瘤生长体积明显大于对照组[（445.3 ±43.2） mm3比（167.8 ±22.9） mm3,P＜0.05）,干预组肿瘤生长体积相对实验组明显减小[（235.4±16.5）mm3比（445.3±43.2） mm3,P＜0.05];Ang-1及血管标志物CD34在肿瘤组织中分布一致;实验组中Ang-1、CD34的蛋白及mRNA表达明显高于干预组及对照组（P＜0.05）.结论 aHSC可通过分泌Ang-1促进肝细胞癌生长,并促进肝癌血管生成.Objective To investigate the effects of activated hepatic stellate cells （aHSCs） on proliferation and angiogenesis in nude mice hepatocellular carcinoma （HCC） model through secretion of angiopoietin-1.Methods A hepatocellular carcinoma model was established by means of subcutaneous injection on nude mice.In the experimental group,the mixture of LX-2 and HepG2 cells （1∶1,100 μl in total,and 5 × 109/L of both cell lines） were given.In the the intervention group,the mixture of LX-2 （siAng-1） and HepG2 （1∶1,100 μl in total,and 5 × 109/L of both cell lines）.In the control group,only HepG2 cells were given （100 μl in total,5 × 109/L）.The growth of tumors was observed,and the tumors were resected after four weeks.Meanwhile,the protein and mRNA expression levels of Ang-1,Ang-2 and CD34 were detected.Results The tumor size in experimental group was significantly bigger than in control group [（445.3 ± 43.2） mm3 vs.（167.8 ± 22.9） mm3,P ＜ 0.05],and the growth rate was decreased when Ang-1 mRNA was silenced [（445.3 ± 43.2） mm3 vs.（235.4 ± 16.5） mm3,P ＜ 0.05].Ang-1 was consistent to CD34 in distribution,and the expression levels of CD34 and Ang-1 in experimental group were significantly higher than those in the intervention group and control group （both P ＜ 0.05）.Conclusion aHSC can promote both the growth of hepatoma cells and angiogenesis in hepatocellular carcinoma through secretion of angiopoietin-1.

关 键 词：癌 肝细胞 血管生成素-1 血管生成 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]