人类表皮生长因子受体-2/neu小分子干扰RNA对脑膜瘤细胞增殖活性的影响  

Effects of human epidermalgrowth factor receptor-2/neu small interfering RNA-mediated gene silencing on cell proliferative activity in human meningiomas

作　　者：王惟佳 徐姗[1] 王珊珊[1] 胡雪梅[1] 王淳良[2] 梅金红[1] 

机构地区：[1]南昌大学第一附属医院病理科,330006 [2]南昌大学第一附属医院神经外科,330006

出　　处：《中华实验外科杂志》2015年第6期1355-1357,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81260372）;江西省教育厅科学技术研究重点项目（GJJ 11013）;江西省科技厅重大攻关计划资助项目（20114004）

摘　　要：目的 观察人类表皮生长因子受体-2（Her-2）/neu基因对脑膜瘤细胞增殖能力的影响.方法 收集脑膜瘤Her-2阳性患者术后新鲜标本5例,原代培养细胞,用脂质体法干扰细胞Her-2基因后,分别采用反转录-聚合酶链反应（RT-PCR）、Western blot法检测Her-2 mRNA和蛋白的表达;噻唑蓝（MTT）法、流式细胞术检测细胞增殖活性及周期的变化.结果 转染后72 h,干扰组与阴性对照组比较,Her-2 mRNA和蛋白表达水平分别下降56.3％和52.7％,差异有统计学意义（P＜0.05）;干扰组转染后72 h的抑制作用最强,细胞增殖抑制率为50.08％,细胞周期中G0/G1期细胞比例上升18.11％,S期比例下降39.34％,差异有统计学意义（P＜0.05）.结论 Her-2/neu小分子干扰RNA可抑制脑膜瘤细胞的增殖活性.Objective To investigate the effects of human epidermal growth factor receptor-2 （Her-2）/neu gene on cell proliferative activity in human meningiomas.Methods The surgicall resected fresh samples from 5 cases of human meningiomas with Her-2 overexpression were selected,and cells were primarily cultured.The expression of Her-2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blotting respectively.Methyl thiazol tetrazolium （MTT） assay was employed to examined the cell viability.The cell cycle distribution was measured by flow cytometry.Results At 72nd h after transfection,as compared with the silencing group and the negative control group,the Her-2 mRNA and protein expression was reduced by 56.3％ and 52.7％ respectively （P ＜ 0.05）.At 72nd h after transfection,the proliferation inhibition rate was 50.08％.The proportion of cells in G0/G1 phase was increased by 18.11％,and that in S phase decreased by 39.34％ （P ＜0.05）.Conclusion Her-2 small interfering RNA （siRNA）-mediated gene may inhibit the proliferative ablity of human meningiomas.

关 键 词：脑膜瘤 人类表皮生长因子受体-2 RNA干扰 增殖 

分 类 号：R739.45[医药卫生—肿瘤]