乳腺癌组织中DNA甲基转移酶1和雌激素受体α的表达与雌激素受体α甲基化状态的关系及临床意义  

Correlation of expression of DNA methy transferases 1 and estrogen receptor alpha with methylation status of estrogen receptor alpha and its clinical significance in breast cancer

作　　者：张伟杰[1] 宋丽杰[1] 赵瑞华[1] 马志俊[1] 宗红[1] 樊青霞[1] 王留兴[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科,450052

出　　处：《中华实验外科杂志》2015年第6期1394-1397,共4页Chinese Journal of Experimental Surgery

基　　金：河南省医学科技攻关计划资助项目（201203007）

摘　　要：目的 探讨乳腺癌组织中DNA甲基转移酶1（DNMT1）和雌激素受体α（ERα）的表达与ERα甲基化状态的关系及临床意义.方法 采用免疫组织化学链菌素抗生物素蛋白-过氧化物酶（SP）法和反转录-聚合酶链反应（RT-PCR）检测20例正常乳腺组织和112例乳腺癌组织中DNMT1和ERα蛋白及mRNA的表达;甲基化特异性PCR（MS-PCR）检测不同组织中ERα基因启动子的甲基化状态.结果 免疫组织化学染色和RT-PCR结果显示：正常乳腺组织及ERα阳性乳腺癌组织中DNMT1的蛋白及mRNA阳性表达率较低,分别为10.00％、46.05％和15.00％、48.68％;而在ERα阴性乳腺癌组织中表达较高,为81.11％和88.89％,组间比较差异均有统计学意义（P＜0.05）.MS-PCR结果显示：正常乳腺组织、ERα阳性乳腺癌组织、ERα阴性乳腺癌组织中ERα启动子甲基化阳性率分别为10.00％、36.84％和86.11％,组间比较差异有统计学意义（P＜0.05）.DNMT1的表达和ERα的表达呈负相关（P＜0.05）;DNMT1的表达和ERα启动子的甲基化率呈正相关关系（P＜0.05）.结论 在乳腺癌组织中,DNMT1的表达和ERα的表达呈负相关;DNMT1的表达和ERα的甲基化率呈正相关.Objective To investigate the correlation of DNA methy transferases 1 （DNMT1） and estrogen receptor alpha （ERα） expression with ERα methylation and its clinical significance in normal breast specimens and breast cancer specimens.Methods Immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） were used to detect the protein and mRNA expression of DNMT1 and ERα in 20 cases of normal breast specimens and 112 cases of breast cancer specimens.Mutagenically separated polymerase chain reaction （MS-PCR） was used to detect the methylation status of ERα in different specimens.Results Immunohistochemistry and reverse RT-PCR results showed that the positive rate of DNMT1 protein and mRNA was low in normal breast specimens and ERα-positive breast cancer specimens,which was 10.00％ and 46.05％,and 15.00％ and 48.68％ respectively,while it was high in ERα-negative breast cancer specimens,which was 81.11％ and 88.89％ respectively （P ＜ 0.05）.MS-PCR results revealed that the methylation rate of ERα was increased by turns in normal breast specimens,ERα-positive breast cancer specimens and ERα-negative breast cancer specimens,which was 10.00％,36.84％ and 86.11％ respectively （P ＜0.05）.The expression of DNMT1 was positively correlated to the methylation of ERα （P ＜ 0.05）.Conclusion In breast cancer specimens,the protein and mRNA expression of DNMT1 was negatively correlated to the expression of ERα;the expression of DNMT1 was positively correlated to the methylation of CpG island of ERα.

关 键 词：乳腺癌 DNA甲基转移酶1 雌激素受体Α 甲基化 

分 类 号：R737.9[医药卫生—肿瘤]