互隔交链孢霉素对NIH/3T3细胞的基因损伤及DNA聚合酶β表达的影响  

The genetic damage of altenuene alternate to NIH / 3T3 cells and its effects on the expression of DNA polymerase β

作　　者：马艳英 张晓艳[2] 王瑾瑾[3] 赵继敏[2] 

机构地区：[1]郑州大学附属肿瘤医院病理科,450008 [2]郑州大学基础医学院病理生理教研室,450003 [3]河南省中医学院基础医学院卫生统计学教研室,450008

出　　处：《实用医学杂志》2015年第10期1574-1577,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81472324);河南省教育厅科学技术研究重点项目资助计划(编号:14A310011)

摘　　要：目的:研究互隔交链孢霉素(altenuene,ALT)对NIH/3T3细胞的基因损伤作用及对DNA聚合酶β(DNA polymeraseβ,DNApolβ)表达的影响,探讨互隔交链孢霉(Alternaria alternate)致癌机制。方法 :用单细胞凝胶电泳检测不同浓度的ALT对NIH/3T3细胞的基因损伤作用;以半定量RT-PCR及Western-blot方法分别检测DNApolβ在基因及蛋白水平的表达。结果:10.0、20.0、50.0μmol/L的ALT作用于NIH/3T3细胞后,细胞尾长及尾部DNA含量均增加,一定浓度的ALT作用于细胞后DNApolβ在基因及蛋白水平表达均上调,与溶剂对照组相比,差异均有统计学意义(P<0.05)。结论:ALT可引起NIH/3T3细胞基因损伤,且有剂量依赖性,并可导致DNApolβ的表达上调。Objective To study the effect on genetic damage of ahenuene （ALT） and the expression of DNA polymerase β （CDNApolβ） in NIH/3T3 cells, and discuss the carcinogenesis mechanism of ALT. Methods Single cell gel electrophoresis was used to measure genetic damage to NIH/3T3 cells. RT-PCR and Western-blot were used to detect the expressions of DNApolβ on mRNA and protein level respectively. Results The tail length and content of tail DNA were all increased after treatment with ALT of 10.0, 20.0, 50.0 μmol/L, and the difference was statistically significant （P〈O.05）. The expression of DNApolβ was increased at both the mRNA and protein level after treatment by 20.0 μmol/L ALT. Conclusion ALT may cause gene damage even up to doses and induce DNApolβ overexpression in NIH/3T3 cells.

关 键 词：互隔交链孢霉素 DNApolβ 单细胞凝胶电泳 

分 类 号：R772.2[医药卫生—眼科]