纳米二氧化钛对金鱼脑组织的氧化应激损伤  

Oxidative Stress Damage from Nanometer Titanium Dioxide in Goldfish Brain Tissue

作　　者：孙慧群[1] 张群[1] 陈书琴[1] 操璟璟[1] 

机构地区：[1]安庆师范学院资源环境系,安徽安庆246011

出　　处：《环境科学研究》2015年第6期930-935,共6页Research of Environmental Sciences

基　　金：安徽省教育厅自然科学研究项目(044-K08015000009)

摘　　要：为探讨光催化型nano-Ti O2(纳米二氧化钛)对金鱼(Carassius auratus L.)脑组织的氧化应激损伤效应,以5 nm的锐钛型钛白粉为试验材料,分别在ρ(nano-Ti O2)为0、16、32、64、128 mgL下对金鱼进行处理,测定金鱼脑组织MDA(丙二醛)、PC(羰基化蛋白)和H2O2含量,SOD(超氧化物歧化酶)和GPx(谷胱甘肽过氧化物酶)活性以及CuZn-SOD蛋白表达量.结果表明:ρ(nano-Ti O2)从0 mgL升至128 mgL,金鱼脑组织CuZn-SOD活性从1.70×10-3katkg(以鲜质量计,下同)降至0.35×10-3katkg,再回升至0.46×10-3katkg;Mn-SOD活性呈降—升—降趋势,MDA含量从0.85 nmolg升至2.83 nmolg,之后降至2.54nmolg;PC含量一直上升;H2O2含量从4.32μmolg升至9.28μmolg,之后降至8.38μmolg.随着ρ(nano-Ti O2)的加大,CuZnSOD蛋白表达量逐渐减少.研究表明,nano-Ti O2对金鱼脑组织具有很强的氧化损伤作用,MDA与PC含量升高与CuZn-SOD和GPx活性下降相关,H2O2含量升高与GPx活性下降等因素有关.In order to investigate the oxidative damage mechanism of photocatalytic type nano-TiO2 on goldfish brain tissue, a test involving the content of malondialdehyde （MDA） , protein carbonyl （PC） and H2O2 in goldfish brain tissues together with the activity of superoxide dismutase （SOD） and glutathione peroxidase （GPx） and the expression of Cu/Zn-SOD protein was determined after goldfishes were exposed to different nano-TiO2 concentrations of 0, 16, 32, 64 and 128 mg/L. The results showed that the Cu/Zn-SOD activity in goldfish brain tissues decreased from 1.70 × 10^ -3 kat/kg to 0. 35 × 10^-3 kat/kg, and then rebounded to 0. 46 × 10^-3 kat/kg with the nano- TiO2 concentration varying from 0 to 128 mg/L. Meanwhile, the Mn-SOD activity presented a decrease-increase-decrease trend; the MDA content increased from 0.85 to 2.83 nmol/g, and then decreased to 2.54 nmol/g. The PC content increased all the way when the H2O2 content increased from 4.32 to 9.28 μmol/g, and then decreased to 8.38 μmol/g. When the nano-TiO2 concentration increased, the expression of Cu/Zn-SOD decreased gradually. The research indicated that nano-TiO2 strongly affected the oxidative damage in goldfish brain tissues. The increase of the MDA and PC content was associated with the decreasing of the Cu/Zn-SOD and GPx activity, while HE O2 has a bearing on the GPx activity and other factors.

关 键 词：纳米二氧化钛 氧化损伤 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 羰基化蛋白 

分 类 号：X17[环境科学与工程—环境科学]