狂犬病口服疫苗SRV_9毒株基因序列的生物信息学分析  

Bioinformatics Analysis of Gene Sequence of Oral Rabies Vaccine SRV_9 Strain

作　　者：古丽麦拉.卡日 简子健[1] 夏婷婷[1] 苏晓慧[1] 魏玉圆 王伟[1] 吴辉[1] 毛丽萍[1] 翟少华[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《中国畜牧兽医》2015年第6期1355-1361,共7页China Animal Husbandry & Veterinary Medicine

基　　金：新疆农业大学前期资助项目(XJAU201217)

摘　　要：为了解狂犬病口服疫苗SRV9毒株的基因序列与生物学特性的关系,并就其主要抗原位点与国内外现用狂犬病疫苗生产毒株进行比较,明确其作为口服疫苗株的分子基础,本试验通过RT-PCR方法对狂犬病口服疫苗SRV9毒株的N、P、M、G、L基因分别进行克隆,并分别连接于pMD19-T载体,经酶切、测序鉴定后,用DNAStar软件对全基因序列进行分析,并与11株目前生产用狂犬病疫苗株进行基因序列比对分析和主要抗原位点的比较。狂犬病口服疫苗SRV9毒株N、P、M、G、L基因序列与其他毒株的序列同源性为81.8%~100.0%,由全基因组进化树可知,SRV9毒株与所有疫苗株在同一个大的分支内,均属于基因Ⅰ型,其中狂犬病口服疫苗SRV9毒株与ERA、SAD B19、SAG-2等口服疫苗株的进化距离最近,而与疫苗生产毒株aG、RC-HL之间的进化距离则较远。狂犬病口服疫苗SRV9毒株与各疫苗株的糖蛋白不同结构区的氨基酸同源性分析表明,狂犬病口服疫苗SRV9毒株与SAD B19、SAG-2、ERA口服疫苗株的膜外区、跨膜区和膜内区的同源性最高。狂犬病口服疫苗SRV9毒株的基因组结构和抗原基因位点特征均与目前生产用口服疫苗株相似,这为今后狂犬病口服疫苗的研制和通过分子生物学技术构建基因重组弱毒疫苗株的研究提供了试验依据。The study was aimed to understand the relationship between the gene sequence of SRV9 strain of oral rabies vaccine and biological characteristics, compare its major antigen site with oth- er domestic and overseas strains of rabies vaccine, and clear the molecular basis as oral vaccine strain. The SRV9 N, P, M, G and L genes were cloned by RT-PCR, and connected into the pMD19-T vector, respectively, identified by enzyme digestion, sequenced by DNAStar software, and compared its sequences and the main antigen sites with other 11 strains. The results showed that the sequence homologies of SRV9 N, P,M,G and L genes with others were 81. 8% to 100.0% ,the whole genome phylogenetic tree showed that,all in the same big branch,they were belonged to genotype I ,the evolutionary distance among SRV9 and ERA,SAD B19,SAG-2 were near,and were far among SRV9, aG and RC-HL. The amino acid homology analysis showed that the homology was high among SRV9 and SAD B19, SAG-2, ERA in extramembrance, transmem- brane and intramembrane regions. The results would provide experimental basis for the study of gene recombinant attenuated vaccine strain by molecular biology techniques and development of oral rabies vaccine.

关 键 词：狂犬病病毒 SRV9毒株 全基因组 序列分析 

分 类 号：R392[医药卫生—免疫学]