副猪嗜血杆菌四川株生物学特性及药物敏感性研究  

Study on Biological Characteristics and Drug Sensitivity of Haemophilus parasuis Isolated in Sichuan Province

作　　者：于吉锋[1] 王文贵[1] 魏甬[1] 林毅[1] 李兴玉[1] 叶健强[1] 张先惠[1] 李江凌[1] 廖党金[1] 

机构地区：[1]四川省畜牧科学研究院,成都610066

出　　处：《中国畜牧兽医》2015年第6期1566-1570,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家现代农业产业技术体系四川创新团队(SCCXTD-004);四川省农业科技成果转化项目(2013NC0016);四川省公益性科研院所基本科研项目(SASA2012A02;SASA2013A04;SASA2013B04)

摘　　要：为了解副猪嗜血杆菌四川分离株的生物学特性及耐药性情况,本研究采用微生物及分子生物学技术对副猪嗜血杆菌（Hps）四川分离株从生物学特性、16SrRNA基因PCR扩增和耐药性方面进行了研究。结果显示,Hps四川分离株具有一定的微厌氧性,能产生＂卫星现象＂,分离株与其他地区分离株生化特性基本一致;所有分离株均扩增出821bp的特异性16SrRNA基因片段。药物敏感性试验结果显示,分离株对头孢拉定、氨苄西林、头孢氨苄、阿莫西林、头孢克洛、氧氟沙星、头孢噻肟、呋喃妥因具有较高的敏感性,敏感率为75.61%~87.80%;对林可霉素、羧苄青霉素、恩诺沙星、阿米卡星、磺胺甲唑均表现出较高的耐药性,且多重耐药严重,5~8种耐药占比最多,为21.95%~30.49%,有7株可耐受多达13种药物。结果表明,Hps四川分离株存在严重的耐药性现象。The study was aimed to investigate the biological characsteristics and drug sensitive tests of Haernophilus parasuis isolated in Sichuan province. This paper reported the biological characteristics, 16S rRNA gene PCR and drug sensitive test of the isolates by technique of micro- biological and molecular biology. The results showed that the isolates had micro anaerobic and could produce satellite phenomenon, the isolates were just same in biochemical characteristics with field isolates in other area. All the isolates amplified 821 bp 16S rRNA gene. And drug sensitive tests showed that the isolates were highly sensitive to eefradine, ampicillin, cefradine, amoxicillin, cefaclor, ofloxacin, cefotaxime and furadantin, the sensitive rate was 75.61% to 87.80%, but the isolates were high resistance to lincomycin, carbenicillin, enrofloxacin, amikacin and sulfamethox- azole,and multiple-drug resistance was found among the isolates,the number of bacterial strains resistant to antibiotics were more between five and eight, the resisitance strains were 21.95 % to 30.49%,7 isolates were even resistant to 13 antibiotics. The results showed the phenomenon of drug resistance of the isolates was very serious.

关 键 词：副猪嗜血杆菌 生物学特性 16SRRNA基因 药物敏感性 

分 类 号：S858.28[农业科学—临床兽医学]