清热解毒法与益气健脾法对急性期和慢性期溃疡性结肠炎大鼠NO的影响  

The influence of Qingrejiedu Therapy and Yiqijianpi Therapy on the level of NO in serum of ulcerative colitis rats during acute and chronic phase

作　　者：李文静[1] 林燕[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国中西医结合消化杂志》2015年第5期303-306,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金青年科学基金项目(No:81202682)

摘　　要：[目的]阐明益气健脾法和清热解毒法对急性期和慢性期溃疡性结肠炎的影响,为辨证治疗提供依据。[方法]检测溃疡性结肠炎大鼠血清NO水平,通过观察不同治法的治疗效果探讨其治疗UC的抗炎及免疫机制。[结果]造模完成后,模型组急性期血清NO水平显著高于正常组(P<0.01),慢性期有降低趋势(P<0.01),但仍显著高于正常组(P<0.01)。在急性期,血清NO水平清热解毒组显著低于模型组(P<0.01),且与正常组无统计学差异;益气健脾组、西药组高于正常组(P<0.01);在慢性期,清热解毒组、益气健脾组、西药组均低于模型组(P<0.05),其中益气健脾组显著低于模型组(P<0.01),也低于清热解毒组和西药组(P<0.05);益气健脾组NO水平慢性期显著低于其急性期(P<0.01)。[结论]免疫损伤和炎症反应持续存在于UC全病程,急性期尤为突出,慢性期虽然有所减轻但仍然存在。急性期UC以标实为主,湿热瘀毒为其主要病机,所以清热解毒法能更好地发挥抗炎作用,取得明显疗效。慢性期,以正虚为主,正气不足,邪气衰弱,益气健脾法通过提高免疫力来改善免疫损伤情况,达到更好的治疗效果。这为UC的辨证论治提供客观依据。[Objective]To clarify the effect of Qingrejiedu Therapy and Yiqijianpi Therapy on serum NO of acute and chronic ulcerative colitis rats, and to provide the basis for syndrome differentiation treatment. [Methods]Serum NO level of ulcerative colitis rats was detected. The curative effects of different treatment were observed to explore the anti-inflammatory and immune mechanisms for the treatment of UC. [Results]serum NO level of the model group was significantly higher than that of normal group （P〈0.01） during acute phase, which was decreased during chronic phase （P〈0.01）, but still significantly higher than normal group （P〈0.01）. In the acute phase, serum NO level of Qingrejiedu Therapy group was significantly lower than model group （P〈0. 01）, and had no statistical difference compared with normal group. NO level was higher in Yiqijianpi Therapy group and western medicine group compared with normal group （P〈0.01）. In the chronic phase, NO level was lower in Qingrejiedu Therapy group, Yiqijianpi Therapy group and the western medicine group compared with model group （P〈0.05）. However, the level of NO was obviously lower in Yiqijianpi Therapy group compared with model group, which was also lower than Qingrejiedu Therapy group or western medicine group （P〈0.01）. Yiqijianpi Therapy group had a significantly lower NO level in chronic stage than its acute stage （P〈0.01）. [Conclusion]The iramune and inflammation injury continues to exist in the whole course of UC, especially in acute stage. Excessive Biao of dampness and stasis is the main pathogenesis for acute phase UC, thus Qingrejiedu Therapy can play a better anti-inflammatory effect and achieve significant results. In chronic phase, the main pathogenesis is the weakness of vital qi, thus Yiqijianpi Therapy can improve the immunity to promote immune damage and achieve better therapeutic effect. This provides the objective basis for the syndrome differentiation and treatment of UC.

关 键 词：溃疡性结肠炎 一氧化氮 益气健脾 清热解毒 

分 类 号：R574.62[医药卫生—消化系统]