丰富环境联合氟西汀干预对大鼠抑郁样行为及脑内髓鞘碱性蛋白的影响  

Effects of the intervention with enriched environment and fluoxetine on the depression-like behavior and myelin basic protein of brain tissue in rats

作　　者：谷景阳 韩金红[2] 詹和琴 王长虹[1] 刘聪[1] 单筱雯 翟飞[1] 

机构地区：[1]新乡医学院第二附属医院,新乡453002 [2]新乡医学院基础医学院,新乡453002 [3]新乡医学院药学院,新乡453002

出　　处：《中华行为医学与脑科学杂志》2015年第5期402-406,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：河南省基础与前沿技术研究计划项目（142300410025）;新乡医学院高层次人才科研资助项目（08BSKYQD-004）;河南省科技攻关计划项目（112102310211）;新乡医学院科研培育基金资助项目（2013ZD117）;新乡医学院研究生科研创新支持计划资助项目（YJSCX201243Y）

摘　　要：目的 探讨丰富环境联合氟西汀干预对慢性应激所致抑郁大鼠行为学的影响,以及海马区和前额叶区髓鞘碱性蛋白（myelin basic protein,MBP）变化的影响.方法 将50只成年雄性SD大鼠随机分为对照组、氟西汀组、模型组、丰富环境（enriched environment,EE）组和EE＋氟西汀组,后四组在第1～3周接受慢性不可预见性刺激（chronic unpredicted mild stress,CUMS）刺激;第4～6周,氟西汀组、EE组以及EE＋氟西汀组大鼠接受EE和（或）氟西汀干预;采用蔗糖水消耗实验、旷场实验、体质量变化评估大鼠的行为学变化;使用免疫组化法测量大鼠海马各亚区以及前额叶区MBP的含量.结果 第3周末,应激刺激大鼠的各项行为学评估较对照组明显下降,差异有统计学意义（P＜0.05）;第6周末,接受EE和（或）氟西汀干预后的应激大鼠抑郁样行为显著改善,模型组大鼠海马CA1、DG区以及前额叶区MBP含量较对照组差异均有统计学意义（平均光密度：模型组依次为0.199±0.024,0.204±0.021,0.225±0.028;对照组依次为0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05）;氟西汀组、EE组以及EE＋氟西汀组大鼠海马CA1、DG区以及前额叶区的MBP含量较模型组明显升高,差异均有统计学意义（氟西汀组依次为0.259±0.047,0.266±0.052,0.284±0.031;EE组依次为0.257±0.038,0.258±0.042,0.286±0.037;EE＋氟西汀组依次为0.271±0.046,0.279±0.040,0.289±0.041,P＜0.05）.结论 CUMS所致大鼠抑郁样行为的出现与海马CA1、DG区及前额叶区MBP含量的变化有关,氟西汀及EE干预均能逆转大鼠的抑郁样行为,且可使MBP含量恢复正常.Objective To investigate the effects of intervention with the fluoxetine and the enriched environment on chronic stress induced depression behavior of rats,and the changes of myelin basic protein in hippocampus and prefrontal regions.Methods 50 adult male SD rats were randomly divided into control group,fluoxetine group,model group,enriched environment （EE） group and EE plus fluoxetine group.Fluoxetine group,model group,EE group and EE plus fluoxetine group underwent chronic unpredictable stress stimulus in the first to third week,and fluoxetine group,EE group,EE plus fluoxetine group underwent the intervention with EE and （or） fluoxetine in the fourth to sixth week.The changes of behavior in rats were evaluated by sucrose water consumption,open field test and weight changes.The content of MBP in each subregion of hippocampus and prefrontal regions of rats was measured with immunocytochemical methods.Results At the third weekend,the assessed behaviors of stressed rats decreased significantly compared with control group （P＜0.05）;and at the sixth weekend,the behaviors of stressed rats restored after treated with EE and （or） fluoxetine.The content of MBP in the rat hippocampus CA1,DG area and prefrontal area of model group declined clearly compared with control group （mean density of model group orderly：0.199±0.024,0.204±0.021,0.225±0.028;control group orderly：0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05）.The content of MBP in the rat of fluoxetine group,EE group and EE plus fluoxetine group increased obviously compared with model group （fluoxetine group orderly：0.259± 0.047,0.266± 0.052,0.284 ± 0.031;EE group orderly：0.257±0.038,0.258±0.042,0.286±0.037;EE plus fluoxetine group orderly：0.271± 0.046,0.279±0.040,0.289±0.041,P＜0.05）.Conclusion The depression-like behavior of rats induced by chronic unpredictable stress is associated with the change of the content of MBP in hippocampal CA1,DG area and prefrontal area;and the depression-like behavior and the content of

关 键 词：抑郁 髓鞘碱性蛋白 丰富环境 氟西汀 海马 前额叶 

分 类 号：R749.4[医药卫生—神经病学与精神病学]