重症抑郁障碍外周血microRNA的生物信息学分析  

Bioinformatics analysis of microRNAs differently expressed in major depression disorder

作　　者：范惠民[1] 吴文波 牛威 孙欣羊[1] 仲爱芳 赵林[5] 张理义 

机构地区：[1]第二军医大学临床医学院精神卫生系,上海212013 [2]解放军102医院急诊科 [3]解放军102医院康复科 [4]解放军102医院检验科 [5]苏州市广济医院 [6]全军心理疾病防治中心

出　　处：《中华行为医学与脑科学杂志》2015年第5期418-422,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：2012年度南京军区医学科技创新课题项目（12MA002）

摘　　要：目的 采用生物信息学方法预测has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498和has-miR-4743的靶基因,并分析其所参与的生物学过程及信号通路,为后续功能研究提供理论依据.方法 应用TargetScan、miRBD及DIANA-microT-CDS预测上述5个miRNA的靶基因,各个miRNA的预测结果分别取交集后,再取其合集,应用FunNet进行功能富集分析和信号通路富集分析.结果 用3个在线数据库得到5个miRNA的靶基因的合集为734个;靶基因所涉及的生物学过程广泛,包括多项与中枢神经系统相关的条目,如皮层发育、轴突导向和延伸、突触传递以及学习和记忆过程等（P＜0.05）;所涉及的信号通路包括轴突导向、谷氨酸能突触、Wnt信号通路、ErbB信号通路、mTOR信号通路、VEGF信号通路等与重症抑郁障碍存在密切联系;has-miR-26b、has-miR-1972、has-miR-4498等3个miRNA在重症抑郁障碍中可能起着更加重要的作用.结论 has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498和has-miR-4743可能与重症抑郁障碍的发病机制密切相关.Objective To predict the target genes and function of has-miR-26b,has-miR-1972,has-miR4485,has-miR-4498,and has-miR-4743 by bioinformatics analysis,and provide the theoretical basis for the further research.Methods The targets of the five microRNAs were predicted by Target Scan,miRBD,and DIANA-microT-CDS,and the result were analyzed by gene ontology and pathway analysis using FunNet.Results 734 predicted targets were obtained by finding the intersected genes of Target Scan,miRBD,and DIANA-microT-CDS.GO analysis showed that biological processes regulated by the differentially expressed microRNAs included diverse terms,among which some terms （e.g.,central nervous system development,neuron differentiation,axonogenesis,synaptic transmission,learning,and memory,etc.） had direct relationship with the central nervous system and brain functions.The pathway analysis showed that a significant enrichment in several pathways related to neuronal brain function,such as axon guidance,glutamatergic synapse,Wnt signaling pathway,mTOR signaling pathway,VEGF signaling pathway,etc.Among the five microRNAs,has-miR-26b,has-miR-1972,has-miR-4498 might have more important regulatory functions.Conclusion Bioinformatic analysis indicates that has-miR-26b,has-miR-1972,has-miR-4485,has-miR-4498,and has-miR-4743 are closely related to the mechanism and pathogenesis of major depressive disorder.

关 键 词：niRNA 重症抑郁障碍 靶基因 生物信息学 

分 类 号：R749.4[医药卫生—神经病学与精神病学]