广东省不同亚型AIDS病人HIV-1 gp120 V3环基因变异及其利用辅助受体的情况  

Gene mutation in V3 loop of HIV-1 gpl20 and use of co-receptor among AIDS patients with different subtypes in Guangdong province

作　　者：饶嫚 蔡卫平[1] 魏绍静[1] 肖桂娥[1] 钟剑波[1] 

机构地区：[1]广州医科大学附属广州市第八人民医院传染病研究所,广州510060

出　　处：《中国艾滋病性病》2015年第5期354-357,共4页Chinese Journal of Aids & STD

基　　金：广东省科技计划项目(2012B031800336);十二五国家科技重大专项(2012ZX10001003);广州市科技计划项目(NO2011J4300092)~~

摘　　要：目的了解广东省艾滋病病毒（HIV）主要流行亚型及V3环氨基酸的变异特点,并分析病毒株利用辅助受体的情况。方法收集2013-2014年期间,在广州市第八人民医院住院的艾滋病（AIDS）病人的血浆256份,巢式聚合酶链反应（Nested-PCR）方法扩增env基因C2-C3（HBX2：6972~7365）区并直接测序,用Geen tool软件将基因序列翻译为氨基酸序列进行比对和分析。结果共有214例（83.6%）扩增成功,其中143例（66.8%）为CRF01_AE;45例（21.0%）为B/C重组型;26例（12.1%）为CRF01_AE/B亚型。V3环顶端四肽基序,73.4%（157/214）为GPGQ,11.7%（25/214）为GPGR,8.9%（19/214）为GPGK,2.3%（5/214）为GQGQ,其他为（GPGG、GLGH、GPGS、GPGH）3.7%（8/214）。使用国际上普遍应用的4种软件（PSSMx4r5、SAAC＋BLAS、PSSMsisi、Geno2pheno）分别对V3环顶端四肽基序利用辅助受体的情况进行评估。综合评估结果显示,132例（61.7%）利用CCR5,67例（31.3%）利用CXCR4。在15例（7.0%）无辅助受体评估结果的病例中,14例为CRF01_AE,1例为CRF01_AE/B。研究还发现,71.1%（32/45）B/C重组型的V3四肽基序单一使用PSSMsisi软件的评估结果,与4种软件综合评估结果的一致率为100%。结论目前广东省HIV-1的主要流行株仍是CRF01_AE,其V3环氨基酸高度变异,顶端四肽基序主要是GPGQ。对毒株利用辅助受体的情况,宜采取国际普遍使用的4种软件进行综合分析。Objective This study is to understand the amino acid variation of Env gene V3 loop of the main HIV-1discordant in Guangdong province,and to analyze the receptor of these virus strains.Method These plasma samples were collected from 232HIV/AIDS patients hospitalized in the 8th People Hospital of Guangzhou during 2013-2014.Reverse transcription-nested polymerase chain reaction（RT-nPCR）was performed to amplify Env genes of HIV C2-C3region（HBX2：6972-7365）and sequenced directly.Then these amino acid sequences were translated by Geen tool software to compare and analyze.Result A total of 214 sequences were amplified successfully.It showed that 143（66.8%）samples were CRF01_AE,45（21.0%）samples were B/C recombinant,26（12.2%）samples were B.The analysis of V3 loop amino acid sequence revealed that 73.4%（157/214）were GPGQ,11.7%（25/214）were GPGR,8.9%（19/214）were GPGK,2.3%（5/214）were GQGQ,and other（GPGG GLGH GPGS GPGH）were 3.7%（8/214）.At last,to use four kinds of commonly used software such as PSSMx4r5,SAAC＋BLAS,PSSMsisi,Geno2pheno[coreceptor]in the world to predict which co-receptor can be used.The comprehensive evaluative results displayed that132samples（61.7%）used CCR5co-receptor;67samples（31.3%）used CXCR4co-receptor;15samples had no assessment,of which 14 samples were CRF01-AE,1sample is CRF01_AE/B.The study also found that 32samples（71.1%）B/C recombinant had the same assessment between using PSSMsisi software and using four kinds of software for comprehensive analysis.Conclusion Recently the major epidemic strain was CRF01-AE.The V3 loop amino acids are the hypervariable region.The top tetrapeptide of V3 loop amino acid was mainly GPGQ.For virus strains using co-receptor is appropriate to take four kinds of internationally commonly used software for comprehensive analysis.

关 键 词：艾滋病病毒Ⅰ型 辅助受体 V3环顶端四肽 CCR5 CXCR4 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]