牦牛肠源Lactobacillus acidophilus L3对其肠道分泌型免疫球蛋白A及免疫相关因子的影响  

Effect of Intestinal Lactobacillus acidophilus L3 on the SIg A and Immune Factors of Small Intestine in Yaks

作　　者：祁珊珊[1] 郑红星[2,3] 

机构地区：[1]陕西理工学院维生素D生理与应用研究所,陕西汉中723000 [2]陕西理工学院生物科学与工程学院,陕西汉中723000 [3]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室和甘肃省新兽药工程重点实验室,兰州730050

出　　处：《四川动物》2015年第3期411-414,共4页Sichuan Journal of Zoology

基　　金：陕西省教育厅科学研究计划(自然科学专项)项目2013JK0721;陕西理工学院人才启动院项目SLGQD13-19;陕西理工学院人才启动院项目SLGQD14-01;农业部兽用药物创制重点实验室;甘肃省新兽药工程重点实验室开放课题1610322011011

摘　　要：为了研究牦牛肠源Lactobacillus acidophilus L3（嗜酸乳杆菌）对其肠道分泌型免疫球蛋白A（SIg A）及免疫相关因子的影响,将10头健康牦牛（2～2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠样品,ELISA检测试验组和对照组十二指肠、空肠、回肠中SIg A、白细胞介素-2（IL-2）、IL-4、IL-6、干扰素γ（IFN-γ）的含量。研究结果表明,L.acidophilus L3可有效增加试验组牦牛肠道SIg A的分泌量（P〈0.05）,组内比较发现SIg A在回肠含量最高,其次为空肠和十二指肠。IL-2、IL-4、IL-6在试验组小肠中的表达量较对照组显著增加（P〈0.05）,试验组小肠IFN-γ的表达量较对照组降低（P〈0.05）。证实L.acidophilus L3可提高牦牛肠道的黏膜免疫功能。In order to study the effects of Lactobacillus acidophilus L3 on the intestinal SIgA and immune factors of yaks, 10 yaks aged from 2 to 2.5 years were used in this study, and 5 of which were fed daily with L. acidophilus L3 （ a concen- tration of 2 × 10^9CFU · kg^-1 feed）, while the others were conventional feed without adding L. acidophilus L3. Small intesti- nal samples were collected on day 28, and the content of SIgA, IL-2, IL-4, IL-6, IFN-γ in duodenum, jejunum, and ileum were detected by ELISA. The results showed that the content of intestinal SIgA was significantly increased in the probiotic group compared with the control （ P 〈 0.05 ）. The highest content of SIgA was found in ileum followed by jejunum and duo- denum. The content of intestinal IL-2, IL-4 and IL-6 were also significantly increased in the probiotic group compared with the control （P 〈 0.05）. The content of intestinal IFN-γ was decreased in the probiotic group compared with the control （ P 〈 0.05 ）. These results indicated that L. acidophilus L3 can effectively improve the intestinal mucosal immune function of yaks by increasing the levels of intestinal SIgA, IL-2, IL-4 and IL-6.

关 键 词：牦牛 LACTOBACILLUS ACIDOPHILUS L3 小肠 SIGA 免疫相关因子 

分 类 号：R392[医药卫生—免疫学]