长链非编码RNA BANCR对非小细胞肺癌增殖和侵袭的影响  

Down-regulated long noncoding RNA BANCR promotes cell proliferation and invasion in non-small cell lung cancer

作　　者：陈志强[1] 尹凌帝 孙倩[1] 刘博巽 秦宇 孙明[3] 德伟[3] 刘志军[3] 

机构地区：[1]南京医科大学第一临床医学院,江苏南京210029 [2]正大天晴药业南京研究所生物药室,江苏南京210042 [3]南京医科大学生物化学与分子生物学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2015年第5期650-655,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(81070620)

摘　　要：目的 :探讨长链非编码RNA BANCR在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织及细胞系中的表达水平,以及BANCR对NSCLC细胞增殖和侵袭的影响。方法:用定量PCR技术检测NSCLC组织及细胞系中BANCR的表达水平;通过转染pc DNA-BANCR上调BANCR的表达水平,并通过q PCR检测转染效率。用Transwell实验检测上调BANCR的水平对SPC-A1细胞和A549细胞增殖和侵袭能力的影响,Western blot检测这2种细胞中上调BANCR的水平对上皮-间质转化(epithelial-mesenchymal transition,EMT)标志物上皮型钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)表达水平的影响。结果 :相比正常肺组织及细胞,在NSCLC组织和细胞中BANCR的表达出现显著下调。MTT实验显示,上调BANCR的表达能降低SPCA1细胞和A549细胞的增殖和侵袭能力。BANCR过表达能通过上调E-cadherin表达并下调Vimentin表达,影响EMT。结论:BANCR可通过影响EMT,抑制NSCLC细胞的增殖和侵袭。Objective：To investigate the expression level of long noncoding RNA BANCR in non-small cell lung cancer （NSCLC） tissues and cell lines, and to study the effect of BANCR on NSCLC cell proliferation and invasion. Methods：Quantitative PCR was performed to detect the relative expression of BANCR in NSCLC cell lines and tissues, pCDNA-BANCR was transfected into SPC-A1 cells and A549 cells to up-regulate BANCR expression. Transwell assays were performed to detect the effect of BANCR on NSCLC proliferation and invasion. Western blotting was used to detect the expression level of E-cadherin and Vimentin, markers of epithelial- mesenchymal transition. Results：This study showed that BANCR was lowly expressed both in NSCLC samples and cell lines compared with their corresponding normal tissues and cell lines. MTT assays indicated that up-regulated BANCR inhibited the proliferation and invasion of SPC-A1 and A549 cells. BANCR overexpression elevated the expression of E-cadherin and suppresed the level of Vimentin,thus influencing epithelial-mesenchymal transition. Conclusion： BANCR promotes NSCLC cell proliferation and invasion by affecting epithelial-mesenchymal transition.

关 键 词：非小细胞肺癌 长链非编码RNA BANCR 细胞增殖 侵袭 上皮-间质转化 

分 类 号：R734.2[医药卫生—肿瘤]